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CD46 Antibody (MA5-29116) in ICC/IF
Immunofluorescence staining of CD46 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CD46 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29116, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
产品信息
MA5-29116
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
001
抗原
Recombinant Human CD46 protein (Met1-Asp328)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2785042
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human CD46.
靶标信息
CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: antigen identified by monoclonal antibody TRA-2-10; CD46; CD46 antigen, complement regulatory protein; CD46 molecule, complement regulatory protein; complement membrane cofactor protein; measles virus receptor; Membrane cofactor protein; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MGC26544; TLX; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen
基因别名: AHUS2; CD46; MCP; MIC10; TLX; TRA2.10
UniProt ID: (Human) P15529
Entrez Gene ID: (Human) 4179
adaptive immune response
T cell mediated immunity
proteolysis
complement activation, classical pathway
single fertilization
regulation of Notch signaling pathway
positive regulation of gene expression
negative regulation of gene expression
regulation of complement activation
interleukin-10 production
positive regulation of interleukin-10 production
sequestering of extracellular ligand from receptor
positive regulation of T cell proliferation
negative regulation of catalytic activity
positive regulation of memory T cell differentiation
innate immune response
positive regulation of regulatory T cell differentiation
negative regulation of complement activation
viral entry into host cell
positive regulation of transforming growth factor beta production
