Invitrogen

CD49a Polyclonal Antibody

CD49a Antibody in Immunocytochemistry (ICC/IF)

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CD49a Antibody (PA5-47763) in ICC/IF

Immunofluorescence analysis of CD49a was performed using 70 % confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD49a Polyclonal Antibody (Product # PA5-47763) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A11015),... View More

CD49a Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD49a Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target owing to their inherent genetic constitution. Relative expression of CD49a was observed in HeLa, SH-SY5Y, Hep G2, Mouse and Rat kidney tissue and undetected in K-562, MCF7 and Mouse mammary gland using Anti-CD49a Polyclonal Antibody (Product # PA5-47763) in Western Blot. {RE}

产品信息

PA5-47763

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

免疫组化 (IHC)

- -

免疫组化（石蜡） (IHC (P))

5-15 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

2.5 µg per million cells

产品规格

种属反应

Human

宿主/亚型

Sheep / IgG

分类

Polyclonal

类型

Antibody

抗原

CHO-derived recombinant human Integrin alpha 1/CD49a Phe29-Pro1141

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2608461

产品详细信息

In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 2, rhIntegrin alpha 11, recombinant mouse (rm) Integrin alpha 2, and rmIntegrin alpha 11 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD49 antigen-like family member A; CD49a; integrin alpha 1; Integrin alpha-1; integrin, alpha 1; Laminin and collagen receptor; very late activation protein 1; VLA-1

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基因别名: CD49a; ITGA1; VLA1

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UniProt ID: (Human) P56199

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Entrez Gene ID: (Human) 3672

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功能

receptor binding protein binding collagen binding protein phosphatase binding metal ion binding collagen binding involved in cell-matrix adhesion integrin

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