Invitrogen

CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), FITC, eBioscience™

CD51/CD61 (Integrin alpha v beta 3) Antibody in Flow Cytometry (Flow)

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CD51/CD61 (Integrin alpha v beta 3) Antibody (11-0519-42) in Flow

Staining of the M21 cell line with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD51/CD61 (Integrin alpha/beta 3) FITC (filled histogram). Total viable cells were used for analysis.

CD51/CD61 (Integrin alpha v beta 3) Antibody in Immunocytochemistry (ICC/IF)

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产品信息

11-0519-42

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

5 µL (1 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

23C6

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1907402

产品详细信息

Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported: The 23C6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 23C6 antibody has been pre-titrated and tested by flow cytometric analysis of human melanoma cell line and peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Nintedanib-αVβ6 Integrin Ligand Conjugates Reduce TGFβ-Induced EMT in Human Non-Small Cell Lung Cancer.

International journal of molecular sciences

Andreucci E,Bugatti K,Peppicelli S,Ruzzolini J,Lulli M,Calorini L,Battistini L,Zanardi F,Sartori A,Bianchini F

11-0519-42 was used in Flow cytometry/Cell sorting to highlight αvβ6 receptors as privileged access points for dual-targeting molecular conjugates engaged in an efficient and precise strategy against non-small cell lung cancer.

Thu Jan 12 00:00:00 UTC 2023

Effect of Denosumab on the Change of Osteoclast Precursors Compared to Zoledronate Treatment in Postmenopausal Women with Osteoporosis.

Journal of bone metabolism

Kong SH,Kim JH,Kim SW,Jeong AJ,Lee SH,Ye SK,Shin CS

11-0519-42 was used in Flow cytometry/Cell sorting to conclude that denosumab and zoledronate treatments induced similar changes in OC precursors.

Sun May 01 00:00:00 UTC 2022

Targeting Ultrasmall Gold Nanoparticles with cRGD Peptide Increases the Uptake and Efficacy of Cytotoxic Payload.

Nanomaterials (Basel, Switzerland)

Perrins RD,McCarthy LA,Robinson A,Spry KL,Cognet V,Ferreira A,Porter J,Garc¿a CE,Rodriguez MÁ,Lopez D,Perera I,Conlon K,Barrientos A,Coulter T,Pace A,Hale SJM,Ferrari E,Bachrati CZ

11-0519-42 was used in flow cytometry to combine the versatility of ultrasmall gold nanoparticles with the target selectivity of cRGD peptide for the directed delivery of a cytotoxic payload in a novel design.

Tue Nov 15 00:00:00 UTC 2022

High Density Display of an Anti-Angiogenic Peptide on Micelle Surfaces Enhances Their Inhibition of αvβ3 Integrin-Mediated Neovascularization In Vitro.

Nanomaterials (Basel, Switzerland)

Nagaraj R,Stack T,Yi S,Mathew B,Shull KR,Scott EA,Mathew MT,Bijukumar DR

11-0519 was used in Flow cytometry/Cell sorting to investigate the effect of an anti-angiogenic peptide coating on micelle surfaces on neovascularization.

Sun Mar 22 00:00:00 UTC 2020

High Density Display of an Anti-Angiogenic Peptide on Micelle Surfaces Enhances Their Inhibition of αvβ3 Integrin-Mediated Neovascularization In Vitro.

Nanomaterials (Basel, Switzerland)

Nagaraj R,Stack T,Yi S,Mathew B,Shull KR,Scott EA,Mathew MT,Bijukumar DR

11-0519 was used in Flow cytometry/Cell sorting to investigate the effect of an anti-angiogenic peptide coating on micelle surfaces on neovascularization.

Sun Mar 22 00:00:00 UTC 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 UTC 2019

An Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma In Vitro and In Vivo.

Molecular therapy. Nucleic acids

Cen B,Wei Y,Huang W,Teng M,He S,Li J,Wang W,He G,Bai X,Liu X,Yuan Y,Pan X,Ji A

11-0519 was used in Immunocytochemistry-immunoflourescence to reveal a great translational potential of biRGD-siPIK3CB conjugate as a novel molecule for glioblastoma therapy.

Fri Dec 07 00:00:00 UTC 2018

An Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma In Vitro and In Vivo.

Molecular therapy. Nucleic acids

Cen B,Wei Y,Huang W,Teng M,He S,Li J,Wang W,He G,Bai X,Liu X,Yuan Y,Pan X,Ji A

11-0519 was used in Immunocytochemistry-immunoflourescence to reveal a great translational potential of biRGD-siPIK3CB conjugate as a novel molecule for glioblastoma therapy.

Fri Dec 07 00:00:00 UTC 2018

Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.

Immunology

Swindle EJ,Brown JM,Rådinger M,DeLeo FR,Metcalfe DD

11-0519 was used in Flow cytometry/Cell sorting to investigate the effect of interferon-γ on the biological responses of human mast cells to Staphylococcus aureus.

Sun Nov 01 00:00:00 UTC 2015

Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro.

Human reproduction (Oxford, England)

Green CJ,Fraser ST,Day ML

11-0519 was used in Flow cytometry/Cell sorting to study an increase in apical fibronectin in blastocysts mediated by insulin-like growth factor to increase blastocyst attachment to endometrial epithelial cells in vitro.

Sun Feb 01 00:00:00 UTC 2015

Tumor-targeted in vivo gene silencing via systemic delivery of cRGD-conjugated siRNA.

Nucleic acids research

Liu X,Wang W,Samarsky D,Liu L,Xu Q,Zhang W,Zhu G,Wu P,Zuo X,Deng H,Zhang J,Wu Z,Chen X,Zhao L,Qiu Z,Zhang Z,Zeng Q,Yang W,Zhang B,Ji A

11-0519 was used in Flow cytometry/Cell sorting to assess the anti-tumour potential of an siRNA molecule targetting VEGFR2 conjugated to cRGD peptide.

Wed Oct 01 00:00:00 UTC 2014

Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.

Arthritis research & therapy

Sekine C,Koyanagi A,Koyama N,Hozumi K,Chiba S,Yagita H

11-0519 was used in Flow cytometry/Cell sorting to determine the role of individual Notch receptors and ligands in osteoclastogenesis..

Mon Mar 05 00:00:00 UTC 2012

生物信息学

蛋白别名: antigen CD61; antigen identified by monoclonal antibody L230; CD51; CD61; GPIIIa; integrin alpha V; integrin alpha-5 beta-3; Integrin alpha-V; integrin alphaVbeta3; integrin beta 3; Integrin beta-3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); Platelet membrane glycoprotein IIIa; Vitronectin receptor; Vitronectin receptor subunit alpha

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基因别名: BDPLT16; BDPLT2; CD51; CD61; GP3A; GPIIIa; GT; ITGAV; ITGB3; MSK8; VNRA; VTNR

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UniProt ID: (Human) P06756, (Human) P05106

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Entrez Gene ID: (Human) 3685, (Human) 3690

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功能