Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), Functional Grade, eBioscience™

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

图: 1 / 11

CD54 (ICAM-1) Antibody (16-0542-85) in Flow

Staining of C57Bl/6 splenocytes with 0.25 µg of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD54 (ICAM-1) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85).Cells in the lymphocyte gate were used for analysis.

CD54 (ICAM-1) Antibody in Immunocytochemistry (ICC/IF)

CD54 (ICAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD54 (ICAM-1) Antibody in Immunohistochemistry (IHC)

CD54 (ICAM-1) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

CD54 (ICAM-1) Antibody in Western Blot (WB)

CD54 (ICAM-1) Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

产品信息

16-0542-85

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-Dependent

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

中和实验 (Neu)

Assay-Dependent

功能检测 (Functional)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioKAT-1 (KAT-1, KAT1)

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_529548

产品详细信息

Description: The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), which is a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, and expression increases in response to inflammatory cytokines. ICAM-1 binds to LFA-1 and this interaction is required for the transendothelial migration of T cells. ICAM-1-deficient mice are viable, however the migration of leukocytes to sites of inflammation is reduced leading to impaired immune and inflammatory responses. Based on the regulated expression of ICAM-1, it has been suggested that ICAM-1 may increase leukocyte extravasation at sites of inflammation, whereas the constitutively high expression of ICAM-2 mediates leukocyte traffic into non-inflamed tissue. The eBioKAT-1 monoclonal antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody.

Applications Reported: This eBioKAT-1 (KAT-1, KAT1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KAT-1 monoclonal antibody has also been reported to inhibit the in vivo ICAM-1-mediated migration of leukocytes.

Applications Tested: This eBioKAT-1 (KAT-1, KAT1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Spatially targeted brain cancer immunotherapy with closed-loop controlled focused ultrasound and immune checkpoint blockade.

Science advances

Lee H,Guo Y,Ross JL,Schoen S,Degertekin FL,Arvanitis C

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0542-81) in Immunohistochemistry

Wed Nov 16 00:00:00 UTC 2022

Thrombin Inhibition Prevents Endothelial Dysfunction and Reverses 20-HETE Overproduction without Affecting Blood Pressure in Angiotensin II-Induced Hypertension in Mice.

International journal of molecular sciences

Kij A,Bar A,Przyborowski K,Proniewski B,Mateuszuk L,Jasztal A,Kieronska-Rudek A,Marczyk B,Matyjaszczyk-Gwarda K,Tworzydlo A,Enggaard C,Hansen PBL,Jensen B,Walczak M,Chlopicki S

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0542-81) in Immunocytochemistry

Thu Aug 12 00:00:00 UTC 2021

Effects of Grape Polyphenols on the Life Span and Neuroinflammatory Alterations Related to Neurodegenerative Parkinson Disease-Like Disturbances in Mice.

Molecules (Basel, Switzerland)

Tikhonova MA,Tikhonova NG,Tenditnik MV,Ovsyukova MV,Akopyan AA,Dubrovina NI,Amstislavskaya TG,Khlestkina EK

16-0542-81 was used in Immunohistochemistry to assess the effect of grape polyphenols on the life span of C57BL/6 mice and on behavioral and neuroinflammatory alterations in a transgenic mouse model of Parkinson disease with overexpression of the A53T-mutant human α-synuclein.

Mon Nov 16 00:00:00 UTC 2020

Effects of Grape Polyphenols on the Life Span and Neuroinflammatory Alterations Related to Neurodegenerative Parkinson Disease-Like Disturbances in Mice.

Molecules (Basel, Switzerland)

Tikhonova MA,Tikhonova NG,Tenditnik MV,Ovsyukova MV,Akopyan AA,Dubrovina NI,Amstislavskaya TG,Khlestkina EK

Mon Nov 16 00:00:00 UTC 2020

Dual specificity phosphatase DUSP6 promotes endothelial inflammation through inducible expression of ICAM-1.

The FEBS journal

Hsu SF,Lee YB,Lee YC,Chung AL,Apaya MK,Shyur LF,Cheng CF,Ho FM,Meng TC

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0542-81) in Immunohistochemistry

Tue May 01 00:00:00 UTC 2018

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0542-81) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0542-81) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0542-81) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0542-81) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice.

Molecular biology of the cell

Olaku V,Matzke A,Mitchell C,Hasenauer S,Sakkaravarthi A,Pace G,Ponta H,Orian-Rousseau V

Mon Aug 01 00:00:00 UTC 2011

c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice.

Molecular biology of the cell

Olaku V,Matzke A,Mitchell C,Hasenauer S,Sakkaravarthi A,Pace G,Ponta H,Orian-Rousseau V

Mon Aug 01 00:00:00 UTC 2011

c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice.

Molecular biology of the cell

Olaku V,Matzke A,Mitchell C,Hasenauer S,Sakkaravarthi A,Pace G,Ponta H,Orian-Rousseau V

Mon Aug 01 00:00:00 UTC 2011

c-Met recruits ICAM-1 as a coreceptor to compensate for the loss of CD44 in Cd44 null mice.

Molecular biology of the cell

Olaku V,Matzke A,Mitchell C,Hasenauer S,Sakkaravarthi A,Pace G,Ponta H,Orian-Rousseau V

Mon Aug 01 00:00:00 UTC 2011

生物信息学

蛋白别名: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

查看更多收起

基因别名: CD54; Icam-1; Icam1; Ly-47; MALA-2

查看更多收起

UniProt ID: (Mouse) P13597

查看更多收起

Entrez Gene ID: (Mouse) 15894

查看更多收起

功能

integrin binding protein complex binding

参与通路