Invitrogen

CD56 Recombinant Rabbit Monoclonal Antibody (3H15L12)

CD56 Antibody in Immunocytochemistry (ICC/IF)

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CD56 Antibody (701379) in ICC/IF

Immunofluorescent analysis of NCAM was done on 80% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with NCAM Recombinant Rabbit Monoclonal Antibody (Product # 701379) at 1 µgmL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 fo... View More

CD56 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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产品信息

701379

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

3H15L12

抗原

Recombinant protein corresponding to amino acids 20-170 of human NCAM

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532477

产品详细信息

This antibody is predicted to react with bovine, canine, chimpanzee, feline, non-human primate and rabbit based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis.

The Journal of cell biology

Huang R,Yuan DJ,Li S,Liang XS,Gao Y,Lan XY,Qin HM,Ma YF,Xu GY,Schachner M,Sytnyk V,Boltze J,Ma QH,Li S

701379 was used in Immunohistochemistry-immunofluorescence to study the role of NCAM in mediating neural progenitor cell proliferation and fate decision during corticogenesis.

Mon Jan 06 00:00:00 UTC 2020

HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals.

Journal of cellular physiology

Večeřa J,Bártová E,Krejčí J,Legartová S,Komůrková D,Rudá-Kučerová J,Štark T,Dražanová E,Kašpárek T,Šulcová A,Dekker FJ,Szymanski W,Seiser C,Weitzer G,Mechoulam R,Micale V,Kozubek S

701379 was used in Immunocytochemistry to characterise the importance of co-regulation of H3K9 acetylation by HDAC1 and HDAC3 in the development of the embryonic brain and neuro-differentiation.

Mon Jan 01 00:00:00 UTC 2018

HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals.

Journal of cellular physiology

Večeřa J,Bártová E,Krejčí J,Legartová S,Komůrková D,Rudá-Kučerová J,Štark T,Dražanová E,Kašpárek T,Šulcová A,Dekker FJ,Szymanski W,Seiser C,Weitzer G,Mechoulam R,Micale V,Kozubek S

701379 was used in Immunocytochemistry to characterise the importance of co-regulation of H3K9 acetylation by HDAC1 and HDAC3 in the development of the embryonic brain and neuro-differentiation.

Mon Jan 01 00:00:00 UTC 2018

HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals.

Journal of cellular physiology

Večeřa J,Bártová E,Krejčí J,Legartová S,Komůrková D,Rudá-Kučerová J,Štark T,Dražanová E,Kašpárek T,Šulcová A,Dekker FJ,Szymanski W,Seiser C,Weitzer G,Mechoulam R,Micale V,Kozubek S

701379 was used in Immunocytochemistry to characterise the importance of co-regulation of H3K9 acetylation by HDAC1 and HDAC3 in the development of the embryonic brain and neuro-differentiation.

Mon Jan 01 00:00:00 UTC 2018

HDAC1 and HDAC3 underlie dynamic H3K9 acetylation during embryonic neurogenesis and in schizophrenia-like animals.

Journal of cellular physiology

Večeřa J,Bártová E,Krejčí J,Legartová S,Komůrková D,Rudá-Kučerová J,Štark T,Dražanová E,Kašpárek T,Šulcová A,Dekker FJ,Szymanski W,Seiser C,Weitzer G,Mechoulam R,Micale V,Kozubek S

701379 was used in Immunocytochemistry to characterise the importance of co-regulation of H3K9 acetylation by HDAC1 and HDAC3 in the development of the embryonic brain and neuro-differentiation.

Mon Jan 01 00:00:00 UTC 2018

生物信息学

蛋白别名: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

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基因别名: CD56; E-NCAM; MSK39; N-CAM; N-CAM-1; NCAM; NCAM-1; NCAM-C; NCAM1; NCAMC

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UniProt ID: (Human) P13591, (Mouse) P13595, (Rat) P13596

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Entrez Gene ID: (Human) 4684, (Mouse) 17967, (Rat) 24586

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功能

virus receptor activity Ras guanyl-nucleotide exchange factor activity protein binding heparin binding phosphatase binding LRR domain binding identical protein binding fibroblast growth factor receptor binding cytoskeletal protein binding

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