Invitrogen

CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), FITC, eBioscience™

CD62L (L-Selectin) Antibody in Flow Cytometry (Flow)

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CD62L (L-Selectin) Antibody (11-0629-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0049-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD62L (L-Selectin) FITC (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

11-0629-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (1 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

DREG-56 (DREG56)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10667774

产品详细信息

Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: The DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis.

Applications Tested: The DREG-56 (DREG56) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Induction of memory-like CD8+ T cells and CD4+ T cells from human naive T cells in culture.

Clinical and experimental immunology

Tokumoto Y,Araki Y,Narizuka Y,Mizuno Y,Ohshima S,Mimura T

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 11-0629-42) in Flow Cytometry

Fri Jan 28 00:00:00 UTC 2022

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 11-0629-42) in Flow Cytometry

Sun Apr 24 00:00:00 UTC 2022

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

11-0629 was used in Flow cytometry/Cell sorting to demonstrate that the POC approach is a viable alternative for the generation and use of CAR-T cells, overcoming the limitations of current manufacturing protocols.

Wed Jul 14 00:00:00 UTC 2021

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Wed Jul 14 00:00:00 UTC 2021

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 11-0629-42) in Flow Cytometry

Mon Sep 02 00:00:00 UTC 2019

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Mon Jan 01 00:00:00 UTC 2018

Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.

The Journal of clinical investigation

Rathod KS,Kapil V,Velmurugan S,Khambata RS,Siddique U,Khan S,Van Eijl S,Gee LC,Bansal J,Pitrola K,Shaw C,D'Acquisto F,Colas RA,Marelli-Berg F,Dalli J,Ahluwalia A

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 11-0629-42) in Flow Cytometry

Tue Jan 03 00:00:00 UTC 2017

Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy.

Scientific reports

Feyaerts D,Benner M,van Cranenbroek B,van der Heijden OWH,Joosten I,van der Molen RG

11-0629 was used in Flow cytometry/Cell sorting to immunophenotype lymphocytes from endometrium, term decidua parietalis, and peripheral blood mononuclear cells for direct comparison.

Tue Jun 06 00:00:00 UTC 2017

CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer.

Oncoimmunology

Su S,Zou Z,Chen F,Ding N,Du J,Shao J,Li L,Fu Y,Hu B,Yang Y,Sha H,Meng F,Wei J,Huang X,Liu B

11-0629 was used in Flow cytometry/Cell sorting to study PD-1/PD-L1-mediated immune tolerance of Epstein-Barr virus-associated gastric cancer and provide a new strategy to break the tolerance for the T cell-based adoptive therapy.

Tue Jan 12 00:00:00 UTC 2021

CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients.

Scientific reports

Su S,Hu B,Shao J,Shen B,Du J,Du Y,Zhou J,Yu L,Zhang L,Chen F,Sha H,Cheng L,Meng F,Zou Z,Huang X,Liu B

11-0629 was used in Flow cytometry/Cell sorting to explore the effects of genetic disruption of the gene PD-1 on T cell viability, IFN-γ production and cytotoxicity.

Thu Jan 28 00:00:00 UTC 2016

IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.

Oncoimmunology

Braun M,Ress ML,Yoo YE,Scholz CJ,Eyrich M,Schlegel PG,Wölfl M

11-0629 was used in Flow cytometry/Cell sorting to investigate how IL12, and other inflammatory cytokines, shape effector functions of human T-cells.

Fri Jul 01 00:00:00 UTC 2016

Common gamma chain cytokines promote rapid in vitro expansion of allo-specific human CD8+ suppressor T cells.

PloS one

Yu Y,Zitzner JR,Houlihan J,Herrera N,Xu L,Miller J,Mathew JM,Tambur AR,Luo X

11-0629 was used in Flow cytometry/Cell sorting to demonstrate the use of common γc cytokines in combination with donor APCs as a strategy for generation of donor-specific T cells for immunotherapy.

Mon Aug 27 00:00:00 UTC 2012

Human CD8+CXCR3+ T cells have the same function as murine CD8+CD122+ Treg.

European journal of immunology

Shi Z,Okuno Y,Rifa'i M,Endharti AT,Akane K,Isobe K,Suzuki H

11-0629-42 was used in Flow Cytometry to suggest that human CD8(+)CXCR3(+) T cells are the counterparts of murine CD8(+)CD122(+) Treg.

Sat Aug 01 00:00:00 UTC 2009

Reciprocal CD4+ T-cell balance of effector CD62Llow CD4+ and CD62LhighCD25+ CD4+ regulatory T cells in small cell lung cancer reflects disease stage.

Clinical cancer research : an official journal of the American Association for Cancer Research

Koyama K,Kagamu H,Miura S,Hiura T,Miyabayashi T,Itoh R,Kuriyama H,Tanaka H,Tanaka J,Yoshizawa H,Nakata K,Gejyo F

11-0629 was used in Flow cytometry/Cell sorting to elucidate the immunologic balance induced in small cell lung cancer patients, showing that reciprocal CD4+ T-cell balance of effector and Tregs reflects disease stage.

Sat Nov 01 00:00:00 UTC 2008

生物信息学

蛋白别名: CD62 antigen-like family member L; CD62L; gp90-MEL; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; TQ1

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基因别名: CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; SELL; TQ1

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UniProt ID: (Human) P14151

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Entrez Gene ID: (Human) 6402

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功能

protease binding protein binding heparin binding carbohydrate binding glycosphingolipid binding cell adhesion molecule binding

参与通路

cell adhesion response to ATP regulation of immune response leukocyte migration