Invitrogen

CD64 Monoclonal Antibody (10.1), FITC, eBioscience™

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CD64 Antibody (11-0649-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD64 (Fc gamma Receptor 1) FITC (purple histogram). Cells in the monocyte gate were used for analysis.

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产品信息

11-0649-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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流式细胞分析 (Flow)

5 µL (1 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

10.1

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10544395

产品详细信息

Description: The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative.

Applications Reported: The 10.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 10.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

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Cancers

Prat M,Salon M,Allain T,Dubreuil O,Noël G,Preisser L,Jean B,Cassard L,Lemée F,Tabah-Fish I,Pipy B,Jeannin P,Prost JF,Barret JM,Coste A

Published figure using CD64 monoclonal antibody (Product # 11-0649-42) in Flow Cytometry

Tue Apr 13 00:00:00 UTC 2021

Extracorporeal Hemadsorption versus Glucocorticoids during Cardiopulmonary Bypass: A Prospective, Randomized, Controlled Trial.

Cardiovascular therapeutics

Taleska Stupica G,Sostaric M,Bozhinovska M,Rupert L,Bosnic Z,Jerin A,Ihan A,Klokocovnik T,Podbregar M

11-0649 was used in Flow cytometry/Cell sorting to compare intraoperative hemadsorption and methylprednisolone, with usual care, during complex cardiac surgery on CPB, for inflammatory responses, hemodynamics, and perioperative course.

Mon Jul 06 00:00:00 UTC 2020

Extracorporeal Hemadsorption versus Glucocorticoids during Cardiopulmonary Bypass: A Prospective, Randomized, Controlled Trial.

Cardiovascular therapeutics

Taleska Stupica G,Sostaric M,Bozhinovska M,Rupert L,Bosnic Z,Jerin A,Ihan A,Klokocovnik T,Podbregar M

Mon Jul 06 00:00:00 UTC 2020

Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway.

Cells

Haake K,Neehus AL,Buchegger T,Kühnel MP,Blank P,Philipp F,Oleaga-Quintas C,Schulz A,Grimley M,Goethe R,Jonigk D,Kalinke U,Boisson-Dupuis S,Casanova JL,Bustamante J,Lachmann N

11-0649 was used in Flow cytometry/Cell sorting to report a disease modeling platform to study the role of macrophages in patients with inborn errors of IFN-γ immunity.

Wed Feb 19 00:00:00 UTC 2020

Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies.

mAbs

Hristodorov D,Mladenov R,von Felbert V,Huhn M,Fischer R,Barth S,Thepen T

11-0649 was used in Flow cytometry/Cell sorting to show that targeting M1 macrophages through CD64 can be instrumental in novel strategies for chronic inflammatory conditions.

Mon Jun 13 00:00:00 UTC 2016

Targeting CD64 mediates elimination of M1 but not M2 macrophages in vitro and in cutaneous inflammation in mice and patient biopsies.

mAbs

Hristodorov D,Mladenov R,von Felbert V,Huhn M,Fischer R,Barth S,Thepen T

11-0649 was used in Flow cytometry/Cell sorting to show that targeting M1 macrophages through CD64 can be instrumental in novel strategies for chronic inflammatory conditions.

Mon Jun 13 00:00:00 UTC 2016

Recombinant H22(scFv) blocks CD64 and prevents the capture of anti-TNF monoclonal antibody. A potential strategy to enhance anti-TNF therapy.

mAbs

Hristodorov D,Mladenov R,Brehm H,Fischer R,Barth S,Thepen T

Published figure using CD64 (Fc gamma Receptor 1) monoclonal antibody (Product # 11-0649-42) in Flow Cytometry

Mon Aug 24 00:00:00 UTC 2015

Immunophenotyping in myelodysplastic syndromes can add prognostic information to well-established and new clinical scores.

PloS one

Reis-Alves SC,Traina F,Harada G,Campos PM,Saad ST,Metze K,Lorand-Metze I

11-0649 was used in Flow cytometry/Cell sorting to show that immunophenotypic analysis of myelomonocytic progenitors provides additional prognostic information for clinical scores.

Mon Sep 08 00:00:00 UTC 2014

Intracellular reprogramming of expression, glycosylation, and function of a plant-derived antiviral therapeutic monoclonal antibody.

PloS one

Lee JH,Park DY,Lee KJ,Kim YK,So YK,Ryu JS,Oh SH,Han YS,Ko K,Choo YK,Park SJ,Brodzik R,Lee KK,Oh DB,Hwang KA,Koprowski H,Lee YS,Ko K

11-0649 was used in Flow cytometry/Cell sorting to show that adding an ER retention signal to a plant-derived human anti-rabies mAb expressed in tobacco plants enhances the final mAb yield.

Tue Sep 23 00:00:00 UTC 2014

The guanine nucleotide exchange protein for ADP-ribosylation factor 6, ARF-GEP100/BRAG2, regulates phagocytosis of monocytic phagocytes in an ARF6-dependent process.

The Journal of biological chemistry

Someya A,Moss J,Nagaoka I

11-0649 was used in Flow cytometry/Cell sorting to provide evidence that GEP100 functions in phagocytosis via its role in ARF6-dependent actin remodelling.

Fri Oct 01 00:00:00 UTC 2010

生物信息学

蛋白别名: CD64; CD64 antigen; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc gamma receptor; Fc gamma receptor Ia; Fc-gamma receptor 1; Fc-gamma receptor I A1; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; RP11-196G18.2

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基因别名: CD64; CD64A; FCG1; FCGR1; FCGR1A; FCRI; IGFR1

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UniProt ID: (Human) P12314

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Entrez Gene ID: (Human) 2209

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功能

receptor signaling protein activity protein binding IgG binding immunoglobulin receptor superfamily

参与通路

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent receptor-mediated endocytosis phagocytosis, engulfment immune response signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response interferon-gamma-mediated signaling pathway