Invitrogen

CD69 Monoclonal Antibody (CH/4), PE

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CD69 Antibody (MHCD6904) in Flow

Journal of immunological methods 2014 - Published figure using CD69 monoclonal antibody (Product # MHCD6904) in Flow Cytometry

Product Image Gallery

CD69 Antibody — Figure 4 FACS analysis of CD69 expression. FACS analysis of different cell lines showed Bcr-Abl dependent CD69 expression. Cells were either untreated or treated with 100nM Dasatinib and 1muM Nilotinib for 16h. Percentage of CD69-positive cells is indicated in each histogram. Thirty-six percent of K562 cells showed CD69 expression, which was reduced upon treatment with Dasatinib and Nilotinib CD69 expression to 11% and 12%, respectively (top panel). U937, a Bcr-Abl negative cell line, did not alter their CD69 expression pattern after treatment with either of the drugs (second panel). 26% of untransduced UT-7 cells expressed CD69, whereas Bcr-Abl transduction in these cells increased CD69 expression to 82%. After treatment with either Dasatinib or Nilotinib CD69 expression in untransduced UT-7 remained unchanged, whereas it was decreased in UT7-Bcr-Abl cells (lower two panels). {TM}

产品信息

MHCD6904

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

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流式细胞分析 (Flow)

Assay-Dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Non-human primate, Protozoa, Rhesus monkey

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

CH/4

抗原

Human CD69

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA

内含物

0.1% sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10392698

产品详细信息

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

靶标信息

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Synergy between B cell receptor/antigen uptake and MHCII peptide editing relies on HLA-DO tuning.

Scientific reports

Jiang W,Adler LN,Macmillan H,Mellins ED

MHCD6904 was used in Flow cytometry/Cell sorting to discover the factors involved in critical pathways synergizing antigen-BCR interaction and antigenic peptide-MHCII presentation remaining elusive.

Wed Sep 25 00:00:00 UTC 2019

The impact of Nucleofection® on the activation state of primary human CD4 T cells.

Journal of immunological methods

Zhang M,Ma Z,Selliah N,Weiss G,Genin A,Finkel TH,Cron RQ

MHCD6904 was used in flow cytometry to investigate the effects of transfection on freshly isolated primary CD4 T cell morphology, intracellular calcium levels, cell surface activation markers, and transcriptional activity

Sun Jun 01 00:00:00 UTC 2014

Role of CD8(+) T cells in triggering reversal reaction in HIV/leprosy patients.

Immunology

de Oliveira AL,Amadeu TP,de França Gomes AC,Menezes VM,da Costa Nery JA,Pinheiro RO,Sarno EN

MHCD6904 was used in Flow cytometry/Cell sorting to study the role of CD8(+) T cells in triggering reversal reaction in HIV/leprosy patients.

Sun Sep 01 00:00:00 UTC 2013

Activation of human NK cells by Plasmodium-infected red blood cells.

Methods in molecular biology (Clifton, N.J.)

Horowitz A,Riley EM

MHCD6904 was used in flow cytometry to describe a protocol to assess human NK cell activation following in vitro stimulation with malaria-infected red blood cells.

Wed Jan 30 00:00:00 UTC 2013

Distinct expression patterns of CD69 in mucosal and systemic lymphoid tissues in primary SIV infection of rhesus macaques.

PloS one

Wang X,Xu H,Alvarez X,Pahar B,Moroney-Rasmussen T,Lackner AA,Veazey RS

MHCD6904 was used in immunohistochemistry - frozen section and immunohistochemistry - paraffin section to compare CD69 expression on T cells from the intestine, spleen, lymph nodes, and blood of normal and SIV-infected macaques

Tue Apr 10 00:00:00 UTC 2012

NK cells as effectors of acquired immune responses: effector CD4+ T cell-dependent activation of NK cells following vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Horowitz A,Behrens RH,Okell L,Fooks AR,Riley EM

MHCD6904 was used in flow cytometry to study the molecular mechanisms that regulate the "recall" NK cell response after rabies vaccination

Wed Sep 01 00:00:00 UTC 2010

Activation of human NK cells by malaria-infected red blood cells.

Methods in molecular biology (Clifton, N.J.)

Horowitz A,Riley EM

MHCD6904 was used in Flow cytometry/Cell sorting to describe a protocol to assess activation of human NK cells following in vitro stimulation with malaria-infected red blood cells.

Fri Feb 26 00:00:00 UTC 2010

The chemokine interleukin-8 and the surface activation protein CD69 are markers for Bcr-Abl activity in chronic myeloid leukemia.

Molecular oncology

Hantschel O,Gstoettenbauer A,Colinge J,Kaupe I,Bilban M,Burkard TR,Valent P,Superti-Furga G

Published figure using CD69 monoclonal antibody (Product # MHCD6904) in Flow Cytometry

Wed Oct 01 00:00:00 UTC 2008

The chemokine interleukin-8 and the surface activation protein CD69 are markers for Bcr-Abl activity in chronic myeloid leukemia.

Molecular oncology

Hantschel O,Gstoettenbauer A,Colinge J,Kaupe I,Bilban M,Burkard TR,Valent P,Superti-Furga G

Published figure using CD69 monoclonal antibody (Product # MHCD6904) in Flow Cytometry

Wed Oct 01 00:00:00 UTC 2008

Influence of interleukin-15 on CD8+ natural killer cells in human immunodeficiency virus type 1-infected chimpanzees.

The Journal of general virology

Rodriguez AR,Arulanandam BP,Hodara VL,McClure HM,Cobb EK,Salas MT,White R,Murthy KK

MHCD6904 was used in flow cytometry to determine the contribution of chimpanzee CD3(-) CD8(+) natural killer cells in controlling HIV-1 infection

Thu Feb 01 00:00:00 UTC 2007

Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies.

Molecular pharmacology

Koshiba M,Rosin DL,Hayashi N,Linden J,Sitkovsky MV

MHCD6904 was used in flow cytometry to elucidate the role of A2ARs in the regulation of immune response.

Mon Mar 01 00:00:00 UTC 1999

生物信息学

蛋白别名: Activation inducer molecule; activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; CD69; CD69 antigen (p60, early T-cell activation antigen); EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3

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基因别名: AIM; BL-AC/P26; CD69; CLEC2C; EA1; GP32/28; MLR-3

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UniProt ID: (Human) Q07108

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Entrez Gene ID: (Human) 969

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功能

transmembrane signaling receptor activity calcium ion binding protein binding carbohydrate binding

参与通路

signal transduction cellular response to drug