Invitrogen

CD7 Monoclonal Antibody (4H9), FITC, eBioscience™

图: 1 / 1

CD7 Antibody (11-0078-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0048-42) and Mouse IgG2a kappa Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human CD7 FITC (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

11-0078-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

4H9

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1272076

产品详细信息

Description: This 4H9 monoclonal antibody reacts with human CD7 (also known as Leu-9 or gp40), a 40-kDa transmembrane glycoprotein belonging to the immunoglobulin superfamily. This molecule is expressed on peripheral blood T lymphocytes and natural killer cells, as well as on lymphoid and myeloid lineage precursors during adult hematopoiesis. Capable of providing a costimulatory signal and associating with CD3 and CD45, CD7 is involved in T cell activation.

CD7 has also been detected on numerous T cell-derived leukemias and lymphomias. In fact, because CD7 expression is greater on CD4+ T cells in classical Hodgkin's lymphoma than in benign reactive lymph adenopathy, this molecule may be used as a diagnostic for Hodgkin's lymphoma.

Blocking assays demonstrate that this clone recognizes the same epitope as clone 124-1D1.

Applications Reported: This 4H9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 4H9 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. CD7 contains N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. Further, CD7 is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation. Expression of CD7 is an important marker used in leukemia diagnostics.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Casein kinase 2 controls the survival of normal thymic and leukemic γδ T cells via promotion of AKT signaling.

Leukemia

Ribeiro ST,Tesio M,Ribot JC,Macintyre E,Barata JT,Silva-Santos B

11007842 was used in flow cytometry to identify a role for CK2 in healthy human thymocytes in the selection of the gamma delta T-cell lineage

Sat Jul 01 00:00:00 UTC 2017

Trained immunity in newborn infants of HBV-infected mothers.

Nature communications

Hong M,Sandalova E,Low D,Gehring AJ,Fieni S,Amadei B,Urbani S,Chong YS,Guccione E,Bertoletti A

11-0078 was used in Flow cytometry/Cell sorting to demonstrate that HBV exposure in utero enhances the ability of cord blood immune cells to respond to bacterial infection in vitro.

Wed Mar 25 00:00:00 UTC 2015

Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.

PLoS pathogens

Jo J,Tan AT,Ussher JE,Sandalova E,Tang XZ,Tan-Garcia A,To N,Hong M,Chia A,Gill US,Kennedy PT,Tan KC,Lee KH,De Libero G,Gehring AJ,Willberg CB,Klenerman P,Bertoletti A

11-0078 was used in Flow cytometry/Cell sorting to determine the human liver harbours a network of immune cells able to modulate their responses to different pathogen-associated molecules.

Sun Jun 01 00:00:00 UTC 2014

IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.

Journal of immunology (Baltimore, Md. : 1950)

Tang XZ,Jo J,Tan AT,Sandalova E,Chia A,Tan KC,Lee KH,Gehring AJ,De Libero G,Bertoletti A

11-0078 was used in Flow cytometry/Cell sorting to investigate the function of human mucosal-associated invariant T (MAIT) cells in the human liver and peripheral blood.