Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (MRQ-48), eBioscience™

CD71 (Transferrin Receptor) Antibody in Immunocytochemistry (ICC/IF)

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CD71 (Transferrin Receptor) Antibody (14-0718-93) in ICC/IF

Immunofluorescence analysis of CD71 was performed using 70% confluent log phase THP-1 cells differentiated into Macrophage (M0). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD71(Transferrin Receptor) Mouse Monoclonal Antibody (MRQ-48) (Product # 14-0718-... View More

CD71 (Transferrin Receptor) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD71 (Transferrin Receptor) Antibody in Western Blot (WB)

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产品信息

14-0718-93

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

- -

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

MRQ-48

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

tris, pH 7.3-7.7, with 1% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

AB_2637425

产品详细信息

Description: The MRQ-48 monoclonal antibody recognizes human CD71, also known as the transferrin receptor. CD71 exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed on placental syncytiotrophoblasts, endocrine pancreas, hepatocytes, myocytes, basal keratinocytes, spermatocytes, and erythroid precursors. CD71 also is expressed by immature proliferating cells, at low levels on resting mature lymphocytes, and upregulated after antigen or mitogen stimulation of T and B cells. CD71 expression is used to aid in the identification of erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome.

Applications Reported: This MRQ-48 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunohistochemical staining.

Applications Tested: This MRQ-48 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval. This antibody can be used at less than or equal to 1:100. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。