Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (OX26), APC, eBioscience™

CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

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CD71 (Transferrin Receptor) Antibody (17-0710-82) in Flow

Staining of rat bone marrow cells with Anti-Rat CD45R (B220) PE (Product # 12-0460-82) and 0.125 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or 0.125 µg of Anti-Rat CD71 (Transferrin Receptor) APC (right). Total viable cells were used for analysis.

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产品信息

17-0710-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

OX26

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573153

产品详细信息

Description: The monoclonal antibody OX26 reacts with rat CD71, which is also known as the transferrin receptor. This glycoprotein exists as a homodimer on the cell surface that facilitates the endocytosis of iron by binding to iron-saturated transferrin. CD71 is expressed by proliferating cells of hematopoietic lineages, but not by mature, resting cells or hematopoietic stem cells. Its increased expression, which can be induced by antigen and mitogen stimulation, has been shown to correlate with cell proliferation. Endothelial cells of brain capillaries, cortical neurons, oligodendrocytes, kidney tubules, hepatocytes, pancreatic cells, and muscle cells also express CD71.

The OX26 antibody has been reported to exhibit agonist activity.

Applications Reported: This OX26 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX26 antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Rats provide a superior model of human stress erythropoiesis.

Experimental hematology

Zhang J,Liu Y,Han X,Mei Y,Yang J,Zhang ZJ,Lu X,Ji P

17-0710 was used in Flow cytometry/Cell sorting to establish rat in vitro and in vivo erythropoiesis models that are more appropriate and superior for the study of human stress erythropoiesis than mouse models.