Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD75 Monoclonal Antibody (LN-1), eFluor™ 660, eBioscience™
FIGURE: 1 / 7
CD75 Antibody (50-0759-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and Mouse IgM Isotype Control eFluor® 660 (Product # 50-4752-82) (left) or Anti-Human CD75 eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
50-0759-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgM
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
LN-1
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574175
Product Specific Information
Description: This LN-1 monoclonal antibody reacts with human CD75, a cell surface carbohydrate determinant produced by alpha-2,6-sialytransferase. CD75 is expressed predominantly on mature B cells in peripheral blood and germinal centers, as well as several B cell lines (e.g., Raji, Ramos). CD75 is also expressed on non-immune tissues such as breast, prostate, colon, liver, and kidney. Reports demonstrate that CD75 is a ligand of CD22.
Studies have shown that, depending on fixation and tissue processing, the LN-1 antibody also recognizes astrocytes and microglia in the human brain.
Applications Reported: This LN-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This LN-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Alpha 2,6-ST 1; B-cell antigen CD75; Beta-galactoside alpha-2,6-sialyltransferase 1; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; Sialyltransferase 1; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1; ST6Gal I; ST6GalI
Gene Aliases: SIAT1; ST6GAL1; ST6GalI; ST6N
UniProt ID: (Human) P15907
Entrez Gene ID: (Human) 6480
N-acetylneuraminate metabolic process
humoral immune response
oligosaccharide metabolic process
O-glycan processing
protein N-linked glycosylation via asparagine
positive regulation of mononuclear cell proliferation
response to ethanol
negative regulation of chemotaxis
sialylation
regulation of substrate adhesion-dependent cell spreading
protein sialylation
negative regulation of macrophage apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support