Invitrogen

CD75 Monoclonal Antibody (LN-1), eFluor™ 660, eBioscience™

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CD75 Antibody (50-0759-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and Mouse IgM Isotype Control eFluor® 660 (Product # 50-4752-82) (left) or Anti-Human CD75 eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

50-0759-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

LN-1

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574175

产品详细信息

Description: This LN-1 monoclonal antibody reacts with human CD75, a cell surface carbohydrate determinant produced by alpha-2,6-sialytransferase. CD75 is expressed predominantly on mature B cells in peripheral blood and germinal centers, as well as several B cell lines (e.g., Raji, Ramos). CD75 is also expressed on non-immune tissues such as breast, prostate, colon, liver, and kidney. Reports demonstrate that CD75 is a ligand of CD22.

Studies have shown that, depending on fixation and tissue processing, the LN-1 antibody also recognizes astrocytes and microglia in the human brain.

Applications Reported: This LN-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This LN-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

OCT4 cooperates with distinct ATP-dependent chromatin remodelers in naïve and primed pluripotent states in human.

Nature communications

Huang X,Park KM,Gontarz P,Zhang B,Pan J,McKenzie Z,Fischer LA,Dong C,Dietmann S,Xing X,Shliaha PV,Yang J,Li D,Ding J,Lungjangwa T,Mitalipova M,Khan SA,Imsoonthornruksa S,Jensen N,Wang T,Kadoch C,Jaenisch R,Wang J,Theunissen TW

50-0759-42 was used in Flow cytometry/Cell sorting to mapped the OCT4 interactomes in naive and primed hESCs, revealing extensive connections to mammalian ATP-dependent nucleosome remodeling complexes.

Thu Aug 26 00:00:00 UTC 2021

Probing the signaling requirements for naive human pluripotency by high-throughput chemical screening.

Cell reports

Khan SA,Park KM,Fischer LA,Dong C,Lungjangwa T,Jimenez M,Casalena D,Chew B,Dietmann S,Auld DS,Jaenisch R,Theunissen TW

50-0759-42 was used in Flow Cytometry to elucidate the signaling pathways which lead to the formation of unique naive human embryonic stem cell states.

Tue Jun 15 00:00:00 UTC 2021

Derivation of trophoblast stem cells from naïve human pluripotent stem cells.

eLife

Dong C,Beltcheva M,Gontarz P,Zhang B,Popli P,Fischer LA,Khan SA,Park KM,Yoon EJ,Xing X,Kommagani R,Wang T,Solnica-Krezel L,Theunissen TW

Published figure using CD75 monoclonal antibody (Product # 50-0759-42) in Flow Cytometry

Wed Feb 12 00:00:00 UTC 2020

Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency Regulation.

Cell reports

Bi Y,Tu Z,Zhang Y,Yang P,Guo M,Zhu X,Zhao C,Zhou J,Wang H,Wang Y,Gao S

50-0759 was used in Flow cytometry/Cell sorting to identify a functional surface marker for human naive pluripotency.

Tue Mar 17 00:00:00 UTC 2020

Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States.

Cell stem cell

Collier AJ,Panula SP,Schell JP,Chovanec P,Plaza Reyes A,Petropoulos S,Corcoran AE,Walker R,Douagi I,Lanner F,Rugg-Gunn PJ

Published figure using CD75 monoclonal antibody (Product # 50-0759-42) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2017

生物信息学

蛋白别名: Alpha 2,6-ST 1; B-cell antigen CD75; Beta-galactoside alpha-2,6-sialyltransferase 1; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; Sialyltransferase 1; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1; ST6Gal I; ST6GalI

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基因别名: SIAT1; ST6GAL1; ST6GalI; ST6N

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UniProt ID: (Human) P15907

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Entrez Gene ID: (Human) 6480

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功能

beta-galactoside alpha-2,6-sialyltransferase activity protein binding sialyltransferase activity transferase

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