Invitrogen

CD81 Monoclonal Antibody (1D6-CD81), APC, eBioscience™

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CD81 Antibody (17-0819-42) in Flow

Knockout of CD81 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR661098_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD81 was performed by staining THP-1 CD81 Knock out cells with 1 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ (Product # 17-4714-82, yellow histogram) or 1 µg CD81 Monoclonal Antibody (1D6-CD81), APC, eBioscience™ (Product # 17-0819-42, blue histogram). THP-1 Cas9 control cells were also stained with1 µ... View More

Product Image Gallery

CD81 Antibody — Antibody clone (1D6-CD81) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in 1D6-CD81 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD81 antibody (1D6-CD81). Yellow histogram represents staining with the isotype control. {KO}

产品信息

17-0819-42

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (1 µg)/test

查看6篇已发表文章 6篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Pig

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

1D6-CD81

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11150235

产品详细信息

Description: This 1D6-CD81 monoclonal antibody reacts with human CD81, which is also known as Target of Anti-Proliferative Ab-1 (TAPA-1). A member of the tetraspanin superfamily of transmembrane proteins, CD81 is widely expressed on immune cells such as B, T, and NK cells, monocytes, and eosinophils. Studies suggest that the highest expression of CD81 can be found on germinal center B cells. This protein can also be detected on non-Hodgkin lymphomas and diffuse large B-cell lymphomas. On B cells, CD81 exists in a complex with CD19, CD21, and Leu13. CD81 plays a role in segregating the CD19/CD21-B cell receptor complexes to lipid rafts to activate signal transduction. Finally, CD81 is a receptor for hepatitis C virus.

The 1D6 monoclonal antibody has been reported to induce adhesion and reduce cell proliferation.

Applications Reported: This 1D6-CD81 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1D6-CD81 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Insights into cellular behavior and micromolecular communication in urothelial micrografts.

Scientific reports

Juul N,Willacy O,Mamand DR,Andaloussi SE,Eisfeldt J,Chamorro CI,Fossum M

17-0819-42 was used in Flow Cytometry to demonstrate that increased tissue fragmentation subsequently potentiated the yield of proliferative cells and the cellular expansion potential, which confirms, that the micrografting principles of skin epithelium also apply to uroepithelium.

Mon Aug 21 00:00:00 UTC 2023

Insights into cellular behavior and micromolecular communication in urothelial micrografts.

Scientific reports

Juul N,Willacy O,Mamand DR,Andaloussi SE,Eisfeldt J,Chamorro CI,Fossum M

Mon Aug 21 00:00:00 UTC 2023

Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells.

Journal of applied oral science : revista FOB

Sunartvanichkul T,Arayapisit T,Sangkhamanee SS,Chaweewannakorn C,Iwasaki K,Klaihmon P,Sritanaudomchai H

Published figure using CD81 monoclonal antibody (Product # 17-0819-42) in Flow Cytometry

Fri Apr 14 00:00:00 UTC 2023

uPAR⁺ extracellular vesicles: a robust biomarker of resistance to checkpoint inhibitor immunotherapy in metastatic melanoma patients.

Journal for immunotherapy of cancer

Porcelli L,Guida M,De Summa S,Di Fonte R,De Risi I,Garofoli M,Caputo M,Negri A,Strippoli S,Serratì S,Azzariti A

Sat May 01 00:00:00 UTC 2021

uPAR⁺ extracellular vesicles: a robust biomarker of resistance to checkpoint inhibitor immunotherapy in metastatic melanoma patients.

Journal for immunotherapy of cancer

Porcelli L,Guida M,De Summa S,Di Fonte R,De Risi I,Garofoli M,Caputo M,Negri A,Strippoli S,Serratì S,Azzariti A

Sat May 01 00:00:00 UTC 2021

Open conformation of tetraspanins shapes interaction partner networks on cell membranes.

The EMBO journal

Yang Y,Liu XR,Greenberg ZJ,Zhou F,He P,Fan L,Liu S,Shen G,Egawa T,Gross ML,Schuettpelz LG,Li W

17-0819-42 was used in Western Blotting to investigate if an open conformation is required for tetraspanin-partner interactions to support myriad cellular processes.

Tue Sep 15 00:00:00 UTC 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 UTC 2019

Continuous signaling of CD79b and CD19 is required for the fitness of Burkitt lymphoma B cells.

The EMBO journal

He X,Kläsener K,Iype JM,Becker M,Maity PC,Cavallari M,Nielsen PJ,Yang J,Reth M

17-0819 was used in Flow cytometry/Cell sorting to show that Igβ and CD19 are part of an alternative B-cell signalling module that use continuous ITAM/PI3K signalling to promote the survival of B lymphoma and normal B cells.

Fri Jun 01 00:00:00 UTC 2018

CD81 protein is expressed at high levels in normal germinal center B cells and in subtypes of human lymphomas.

Human pathology

Luo RF,Zhao S,Tibshirani R,Myklebust JH,Sanyal M,Fernandez R,Gratzinger D,Marinelli RJ,Lu ZS,Wong A,Levy R,Levy S,Natkunam Y

Published figure using CD81 monoclonal antibody (Product # 17-0819-42) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2010

生物信息学

蛋白别名: 26 kDa cell surface protein TAPA-1; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin-28; Tetraspanin28; Tspan-28

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基因别名: CD81; CVID6; S5.7; TAPA1; TSPAN28

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UniProt ID: (Human) P60033

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Entrez Gene ID: (Human) 975

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功能

protein binding MHC class II protein complex binding transferrin receptor binding

参与通路