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产品信息
MA1-80209
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Armenian hamster
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
Eat2
抗原
38C13, murine B cell line.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_929499
产品详细信息
This antibody induces homotypic aggregation of B cells and inhibits anti-Ig and IL-4 induced proliferation, and requires the presence of both extracellular loops of TAPA-1 for binding.
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Hamster anti Mouse CD81 antibody, clone Eat2 recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1.
靶标信息
CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 26 kDa cell surface protein TAPA-1; CD 81 antigen; CD81; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); Target of the antiproliferative antibody 1; Tetraspanin28
基因别名: Cd81; Tapa-1; Tapa1; Tspan28
UniProt ID: (Mouse) P35762
Entrez Gene ID: (Rat) 25621, (Mouse) 12520
activation of MAPK activity
phosphatidylinositol biosynthetic process
protein localization
positive regulation of cell proliferation
response to wounding
positive regulation of B cell proliferation
regulation of growth
regulation of cell proliferation
positive regulation of 1-phosphatidylinositol 4-kinase activity
phosphatidylinositol metabolic process
positive regulation of peptidyl-tyrosine phosphorylation
regulation of cell motility
positive regulation of cell growth
