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CD86 Antibody (MA5-30196) in ICC/IF
Immunofluorescence staining of B7-2 in Raji cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD86 Recombinant Rabbit Monoclonal Antibody (103) (Product # MA5-30196, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to.
产品信息
MA5-30196
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
103
抗原
Recombinant Human CD86/B7-2 Protein (Met1-His239)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2785960
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human CD86/B7-2.
靶标信息
CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Activation B7-2 antigen; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2; B70; BU63; CD28 antigen ligand 2; CD86; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; FUN-1; MGC34413; T-lymphocyte activation antigen CD86
基因别名: B7-2; B7.2; B70; CD28LG2; CD86; LAB72
UniProt ID: (Human) P42081
Entrez Gene ID: (Human) 942
response to yeast
adaptive immune response
T cell proliferation involved in immune response
negative regulation of T cell anergy
immune response
cell-cell signaling
aging
positive regulation of cell proliferation
regulation of phosphatidylinositol 3-kinase signaling
T cell costimulation
toll-like receptor 3 signaling pathway
response to interferon-gamma
positive regulation of activated T cell proliferation
T cell activation
B cell activation
response to drug
myeloid dendritic cell differentiation
positive regulation of lymphotoxin A biosynthetic process
positive regulation of interleukin-2 biosynthetic process
positive regulation of interleukin-4 biosynthetic process
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription, DNA-templated
viral entry into host cell
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
defense response to virus
cellular response to lipopolysaccharide
cellular response to metal ion
cellular response to cytokine stimulus
