Invitrogen

CD86 Monoclonal Antibody (GL-1)

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CD86 Antibody (MA1-10299) in Flow

Separation of murine CD86 positive myeloid cells (red-filled) from murine CD86 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of mouse peritoneum fluid cells suspension stained using anti-mouse CD86 (GL-1) purified Monoclonal antibody (Product # MA1-10299) (low endotoxin, concentration in sample 0.6 µg/mL) DAR APC.

CD86 Antibody in Immunocytochemistry (ICC/IF)

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产品信息

MA1-10299

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

Assay-dependent

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免疫细胞化学 (ICC/IF)

Assay-dependent

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流式细胞分析 (Flow)

2 µg/mL

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免疫沉淀 (IP)

Assay-dependent

功能检测 (Functional)

Assay-dependent

其他PubMed (MISC)

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产品规格

种属反应

Mouse

已发表种属

Mouse, Rat

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

GL-1

抗原

LPS-activated CBA/Cs mouse splenic B cells

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_11152324

产品详细信息

This antibody reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Functional Application: Blocking.

靶标信息

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

OX40 agonism enhances PD-L1 checkpoint blockade by shifting the cytotoxic T cell differentiation spectrum.

Cell reports. Medicine

van der Sluis TC,Beyrend G,van der Gracht ETI,Abdelaal T,Jochems SP,Belderbos RA,Wesselink TH,van Duikeren S,van Haften FJ,Redeker A,Ouboter LF,Beyranvand Nejad E,Camps M,Franken KLMC,Linssen MM,Hohenstein P,de Miranda NFCC,Mei H,Bins AD,Haanen JBAG,Aerts JG,Ossendorp F,Arens R

MA1-10299 was used in Flow Cytometry to interrogate whether the landscape of T cell states in the peripheral blood predict responses to combinatorial targeting of the OX40 costimulatory and PD-1 inhibitory pathways.

Tue Mar 21 00:00:00 UTC 2023

TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage.

Frontiers in immunology

Wu X,Zeng H,Xu C,Chen H,Fan L,Zhou H,Yu Q,Fu X,Peng Y,Yan F,Yu X,Chen G

MA1-10299 was used in Immunocytochemistry, Immunohistochemistry to illustrate the correlation between post-SAH (subarachnoid hemorrhage) triggering receptor expressed on myeloid cells 1 (TREM1) plasma levels and SAH severity, show attenuation of neuroinflammation and defects with TREM1 inhibitory peptide LP17, and elucidate associated mechanisms.

Fri Feb 18 00:00:00 UTC 2022

Frontiers in immunology

Wu X,Zeng H,Xu C,Chen H,Fan L,Zhou H,Yu Q,Fu X,Peng Y,Yan F,Yu X,Chen G

Fri Feb 18 00:00:00 UTC 2022

Critical role of post-transcriptional regulation for IFN-γ in tumor-infiltrating T cells.

Oncoimmunology

Salerno F,Guislain A,Freen-Van Heeren JJ,Nicolet BP,Young HA,Wolkers MC

MA1-10299 was used in Flow cytometry/Cell sorting to conclude that post-transcriptional mechanisms could be modulated to potentiate effective T cell therapies in cancer.

Tue Jan 12 00:00:00 UTC 2021

PD-1 Primarily Targets TCR Signal in the Inhibition of Functional T Cell Activation.

Frontiers in immunology

Mizuno R,Sugiura D,Shimizu K,Maruhashi T,Watada M,Okazaki IM,Okazaki T

MA1-10299 was used in Flow cytometry/Cell sorting to indicate that PD-1 primarily targets TCR signal in the inhibition of functional T cell activation.

Tue Aug 04 00:00:00 UTC 2020

C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America

Troegeler A,Mercier I,Cougoule C,Pietretti D,Colom A,Duval C,Vu Manh TP,Capilla F,Poincloux R,Pingris K,Nigou J,Rademann J,Dalod M,Verreck FA,Al Saati T,Lugo-Villarino G,Lepenies B,Hudrisier D,Neyrolles O

MA110299 was used in flow cytometry to find that C-type lectin dendritic cell immunoreceptor is required to modulate lung inflammation and bacterial burden in tuberculosis

Tue Jan 24 00:00:00 UTC 2017

TLR-exosomes exhibit distinct kinetics and effector function.

Scientific reports

Srinivasan S,Su M,Ravishankar S,Moore J,Head P,Dixon JB,Vannberg F

MA110299 was used in immunohistochemistry - frozen section to report the differential effector function by exosomes in communicating the toll like receptor activation state of the original activated cell

Tue Mar 14 00:00:00 UTC 2017

Vitamin D reduces high-fat diet induced weight gain and C-reactive protein, increases interleukin-10, and reduces CD86 and caspase-3.

Pathophysiology : the official journal of the International Society for Pathophysiology

Gomaa AM,El-Aziz EA

MA110299 was used in immunohistochemistry to study the effects of a high-fat diet on the spleen and immune system and to determine the protective effects of chronic treatment with vitamin D

Wed Mar 01 00:00:00 UTC 2017

CXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFNγ production and tumor control.

Oncoimmunology

Veinotte L,Gebremeskel S,Johnston B

MA110299 was used in flow cytometry to study the contribution of CXCR6/CXCL16 interactions in glycolipid-dependent invariant natural killer cell activation and tumor control

Wed Jun 01 00:00:00 UTC 2016

Lymphocytic Microparticles Modulate Angiogenic Properties of Macrophages in Laser-induced Choroidal Neovascularization.

Scientific reports

Tahiri H,Omri S,Yang C,Duhamel F,Samarani S,Ahmad A,Vezina M,Bussières M,Vaucher E,Sapieha P,Hickson G,Hammamji K,Lapointe R,Rodier F,Tremblay S,Royal I,Cailhier JF,Chemtob S,Hardy P

MA110299 was used in flow cytometry to study the ability of lymphocyte-derived microparticles to modulate the gene expression pattern of angiogenesis-related factors in macrophages

Tue Nov 22 00:00:00 UTC 2016

Inhibition of the TNF Family Cytokine RANKL Prevents Autoimmune Inflammation in the Central Nervous System.

Immunity

Guerrini MM,Okamoto K,Komatsu N,Sawa S,Danks L,Penninger JM,Nakashima T,Takayanagi H

MA1-10299 was used in Flow Cytometry to show that receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL) on T cells regulates C-C type chemokine ligand 20 (CCL20) production by astrocytes and T cell localization in the CNS.

Tue Dec 15 00:00:00 UTC 2015

Inflammatory neovascularization during graft-versus-host disease is regulated by αv integrin and miR-100.

Blood

Leonhardt F,Grundmann S,Behe M,Bluhm F,Dumont RA,Braun F,Fani M,Riesner K,Prinz G,Hechinger AK,Gerlach UV,Dierbach H,Penack O,Schmitt-Gräff A,Finke J,Weber WA,Zeiser R

MA1-10299 was used in Flow Cytometry to identify negative regulation of GvHD-related neovascularization by miR-100.

Thu Apr 25 00:00:00 UTC 2013

Targeted inactivation of the tetraspanin CD37 impairs T-cell-dependent B-cell response under suboptimal costimulatory conditions.

Molecular and cellular biology

Knobeloch KP,Wright MD,Ochsenbein AF,Liesenfeld O,Löhler J,Zinkernagel RM,Horak I,Orinska Z

MA1-10299 was used in flow cytometry to generate and characterize mice deficient for CD37.

Tue Aug 01 00:00:00 UTC 2000

生物信息学

蛋白别名: Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD86; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; MGC34413; T-lymphocyte activation antigen CD86

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基因别名: B7; B7-2; B7.2; B70; Cd28l2; Cd86; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2

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UniProt ID: (Mouse) P42082

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Entrez Gene ID: (Mouse) 12524

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功能

receptor binding

参与通路

toll-like receptor signaling pathway immune system process negative regulation of T cell anergy positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation defense response to virus cellular response to lipopolysaccharide