Invitrogen

CD86 (B7-2) Monoclonal Antibody (PO3.1), PE, eBioscience™

CD86 (B7-2) Antibody in Flow Cytometry (Flow)

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CD86 (B7-2) Antibody (12-0861-82) in Flow

Surface staining of LPS-stimulated splenocytes with Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (open histogram) and Anti-Mouse CD86 (B7-2) PE (filled histogram). Total viable cells were used for analysis.

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产品信息

12-0861-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

PO3.1

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465765

产品详细信息

Description: The PO3.1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.

Applications Reported: The PO3.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The PO3.1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Effect of mesenchymal stem cell-derived exosomes on the induction of mouse tolerogenic dendritic cells.

Journal of cellular physiology

Shahir M,Mahmoud Hashemi S,Asadirad A,Varahram M,Kazempour-Dizaji M,Folkerts G,Garssen J,Adcock I,Mortaz E

12-0861 was used in Flow cytometry/Cell sorting to investigate the effects of mesenchymal stem cell-derived exosomes and to understand their role in modulating the mouse tolergenic dendritic cell immune responses.

Thu Oct 01 00:00:00 UTC 2020

Induced pluripotent stem cells reprogrammed from primary dendritic cells provide an abundant source of immunostimulatory dendritic cells for use in immunotherapy.

Stem cells (Dayton, Ohio)

Horton C,Davies TJ,Lahiri P,Sachamitr P,Fairchild PJ

12-0861 was used in Flow cytometry/Cell sorting to investigate if epigenetic memory of iPSC-derived dendritic cells may be productively exploited for the generation of potently immunogenic dendritic cells for immunotherapeutic applications.

Wed Jan 01 00:00:00 UTC 2020

IDO inhibitor synergized with radiotherapy to delay tumor growth by reversing T cell exhaustion.

Molecular medicine reports

Liu M,Li Z,Yao W,Zeng X,Wang L,Cheng J,Ma B,Zhang R,Min W,Wang H

12-0861-82 was used in Flow Cytometry to investigate whether an iindoleamine 2,3‚dioxygenase (IDO) inhibitor synergized with radiotherapy (RT) can further inhibit tumor growth.

Wed Jan 01 00:00:00 UTC 2020

Lymph Node Stromal Cells Generate Antigen-Specific Regulatory T Cells and Control Autoreactive T and B Cell Responses.

Cell reports

Nadafi R,Gago de Graça C,Keuning ED,Koning JJ,de Kivit S,Konijn T,Henri S,Borst J,Reijmers RM,van Baarsen LGM,Mebius RE

12-0861-82 was used in Flow Cytometry to uncover the ability of LNSCs to suppress the early activation of autoreactive immune cells and maintain peripheral tolerance.

Tue Mar 24 00:00:00 UTC 2020

UVB-Induced Tumor Heterogeneity Diminishes Immune Response in Melanoma.

Cell

Wolf Y,Bartok O,Patkar S,Eli GB,Cohen S,Litchfield K,Levy R,Jiménez-Sánchez A,Trabish S,Lee JS,Karathia H,Barnea E,Day CP,Cinnamon E,Stein I,Solomon A,Bitton L,Pérez-Guijarro E,Dubovik T,Shen-Orr SS,Miller ML,Merlino G,Levin Y,Pikarsky E,Eisenbach L,Admon A,Swanton C,Ruppin E,Samuels Y

Published figure using CD86 (B7-2) monoclonal antibody (Product # 12-0861-82) in Flow Cytometry

Thu Sep 19 00:00:00 UTC 2019

Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model.

The Prostate

Sulek JE,Robinson SP,Petrossian AA,Zhou S,Goliadze E,Manjili MH,Toor A,Guruli G

12086182 was used in flow cytometry to study cancer-testis antigen expression in a mouse model of prostate cancer

Wed Mar 01 00:00:00 UTC 2017

Location of tumor affects local and distant immune cell type and number.

Immunity, inflammation and disease

Hensel JA,Khattar V,Ashton R,Lee C,Siegal GP,Ponnazhagan S

12-0861 was used in Flow cytometry/Cell sorting to demonstrate that location of tumour, and not simply the tumour itself, has a definitive role in regulating immune effectors.

Wed Mar 01 00:00:00 UTC 2017

Mesenchymal stromal cell-derived extracellular vesicles rescue radiation damage to murine marrow hematopoietic cells.

Leukemia

Wen S,Dooner M,Cheng Y,Papa E,Del Tatto M,Pereira M,Deng Y,Goldberg L,Aliotta J,Chatterjee D,Stewart C,Carpanetto A,Collino F,Bruno S,Camussi G,Quesenberry P

12-0861 was used in Flow cytometry/Cell sorting to evaluate the capacity of mesenchymal stromal cell-derived extracellular vesicles to rescue murine marrow hematopoietic cells damage following exposure to radiation, showing that damage could be reversed using both murine and human MSC-EVs.

Tue Nov 01 00:00:00 UTC 2016

The Effect of a BSA Conjugate of a Synthetic Hexasaccharide Related to the Fragment of Capsular Polysaccharide of Streptococcus pneumoniae Type 14 on the Activation of Innate and Adaptive Immune Responses.

Frontiers in immunology

Akhmatova NK,Kurbatova EA,Akhmatov EA,Egorova NB,Logunov DY,Gening ML,Sukhova EV,Yashunsky DV,Tsvetkov YE,Nifantiev NE

12-0861 was used in Flow cytometry/Cell sorting to evaluate the effects of immunisation with a synthetic hexasaccharide conjugate, with and without an adjuvant, on TLR activation, dendritic cell maturation, and cytokine production in mice.

Fri Jul 22 00:00:00 UTC 2016

Effect of Chronic Uremia on the Cell Surface Expression of B7 Family Costimulatory Molecules in an HLA-A2 Transgenic Mouse Model of Chronic Kidney Disease.

Comparative medicine

Makidon PE,Smith DM,Groom Ii JV,Cao Z,Landers JJ,Baker JR

12-0861 was used in Flow cytometry/Cell sorting to create a model to investigate the mechanisms involved in chronic uremia-associated immunodeficiency and immunization of chronic kidney disease patient problems.

Sat Aug 01 00:00:00 UTC 2015

Immunogenicity of undifferentiated and differentiated allogeneic mouse mesenchymal stem cells.

Journal of tissue engineering

Mukonoweshuro B,Brown CJ,Fisher J,Ingham E

12-0861 was used in Flow cytometry/Cell sorting to determine that although mesenchymal stem cells possess immunosuppressive properties, they may not be immunoprivileged.

Fri May 09 00:00:00 UTC 2014

The glycosylated Rv1860 protein of Mycobacterium tuberculosis inhibits dendritic cell mediated TH1 and TH17 polarization of T cells and abrogates protective immunity conferred by BCG.

PLoS pathogens

Satchidanandam V,Kumar N,Jumani RS,Challu V,Elangovan S,Khan NA

12-0861 was used in Flow cytometry/Cell sorting to investigate the effect of BCG-TB1860 on both the adaptive and innate immune responses.

Sun Jun 01 00:00:00 UTC 2014

Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells.

PloS one

Ikeda T,Hirata S,Takamatsu K,Haruta M,Tsukamoto H,Ito T,Uchino M,Ando Y,Nagafuchi S,Nishimura Y,Senju S

12-0861 was used in Flow cytometry/Cell sorting to investigate the effect of embryonic stem cell-derived dendritic cells on autoimmunity, showing that suppression of Th1-mediated autoimmunity occurs in NOD and EAE mice.

Thu Dec 17 00:00:00 UTC 2015

The transcription factor STAT5 is critical in dendritic cells for the development of TH2 but not TH1 responses.

Nature immunology

Bell BD,Kitajima M,Larson RP,Stoklasek TA,Dang K,Sakamoto K,Wagner KU,Kaplan DH,Reizis B,Hennighausen L,Ziegler SF

12-0861 was used in Flow cytometry/Cell sorting to investigate how type-2 immunity is affected by STAT5 transcription factor in dendritic cells.

Mon Apr 01 00:00:00 UTC 2013

Thymic stromal lymphopoietin attenuates the development of atherosclerosis in ApoE-/- mice.

Journal of the American Heart Association

Yu K,Zhu P,Dong Q,Zhong Y,Zhu Z,Lin Y,Huang Y,Meng K,Ji Q,Yi G,Zhang W,Wu B,Mao Y,Cheng P,Zhao X,Mao X,Zeng Q

12-0861 was used in Flow cytometry/Cell sorting to examine the phenotype of TSLP-conditioned bone marrow dendritic cells of apolipoprotein E-deficient mice and their capacity to induce the differentiation of Tregs.

Wed Aug 28 00:00:00 UTC 2013

Debridement increases survival in a mouse model of subcutaneous anthrax.

PloS one

Weiner ZP,Boyer AE,Gallegos-Candela M,Cardani AN,Barr JR,Glomski IJ

12-0861 was used in Flow cytometry/Cell sorting to develop a mouse model of debridement to evaluate its effectiveness in the treatment of Bacillus anthracis soft tissue infections.

Mon Jul 23 00:00:00 UTC 2012

Orally administered eicosapentaenoic acid induces rapid regression of atherosclerosis via modulating the phenotype of dendritic cells in LDL receptor-deficient mice.

Arteriosclerosis, thrombosis, and vascular biology

Nakajima K,Yamashita T,Kita T,Takeda M,Sasaki N,Kasahara K,Shinohara M,Rikitake Y,Ishida T,Yokoyama M,Hirata K

12-0861 was used in Flow cytometry/Cell sorting to investigate the effect of the administration of EPA on the regression of atherosclerosis.

Thu Sep 01 00:00:00 UTC 2011

Targeting antigen to mouse dendritic cells via Clec9A induces potent CD4 T cell responses biased toward a follicular helper phenotype.

Journal of immunology (Baltimore, Md. : 1950)

Lahoud MH,Ahmet F,Kitsoulis S,Wan SS,Vremec D,Lee CN,Phipson B,Shi W,Smyth GK,Lew AM,Kato Y,Mueller SN,Davey GM,Heath WR,Shortman K,Caminschi I

12-0861 was used in Flow cytometry to evaluate the targeting of vaccine antigen to dendritic cells via Clec9A for efficient induction of cellular immunity.

Fri Jul 15 00:00:00 UTC 2011

Vaccination with Human Induced Pluripotent Stem Cells Creates an Antigen-Specific Immune Response Against HIV-1 gp160.

Frontiers in microbiology

Yoshizaki S,Nishi M,Kondo A,Kojima Y,Yamamoto N,Ryo A

12-0861 was used in Flow cytometry/Cell sorting to investigate whether human iPSCs (hiPSCs) have the ability to enhance specific immune responses against a HIV-1 antigen in a xenogenic mouse model.

Thu Jul 14 00:00:00 UTC 2011

Oral administration of an active form of vitamin D3 (calcitriol) decreases atherosclerosis in mice by inducing regulatory T cells and immature dendritic cells with tolerogenic functions.

Arteriosclerosis, thrombosis, and vascular biology

Takeda M,Yamashita T,Sasaki N,Nakajima K,Kita T,Shinohara M,Ishida T,Hirata K

12-0861 was used in Flow cytometry/Cell sorting to demonstrate that immune modulation by calcitriol may be a potentially valuable therapeutic approach against atherosclerosis.

Wed Dec 01 00:00:00 UTC 2010

Dibutyl phthalate-induced thymic stromal lymphopoietin is required for Th2 contact hypersensitivity responses.

Journal of immunology (Baltimore, Md. : 1950)

Larson RP,Zimmerli SC,Comeau MR,Itano A,Omori M,Iseki M,Hauser C,Ziegler SF

12-0861 was used in Flow cytometry/Cell sorting to demonstrate a role for TSLP in a Th2 model of contact hypersensitivity in mice.

Mon Mar 15 00:00:00 UTC 2010

Dynamics of lung macrophage activation in response to helminth infection.

Journal of leukocyte biology

Siracusa MC,Reece JJ,Urban JF,Scott AL

Mon Dec 01 00:00:00 UTC 2008

Down-regulation of dendritic cell signaling pathways by Leishmania amazonensis amastigotes.

Molecular immunology

Xin L,Li K,Soong L

12-0861 was used in Flow cytometry/Cell sorting to suggest that La parasites, especially in their intracellular forms, have evolved unique strategies to actively down-regulate early innate signalling events.

Tue Jul 01 00:00:00 UTC 2008

Characterization of Siglec-H as a novel endocytic receptor expressed on murine plasmacytoid dendritic cell precursors.

Blood

Zhang J,Raper A,Sugita N,Hingorani R,Salio M,Palmowski MJ,Cerundolo V,Crocker PR

12-0861 was used in Flow cytometry/Cell sorting to analyse the cellular expression pattern and functional properties of Siglec-H.

Mon May 01 00:00:00 UTC 2006

生物信息学

蛋白别名: Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD86; early T cell costimulatory molecule-1; Early T-cell co-stimulatory molecule 1; Early T-cell costimulatory molecule 1; ETC-1; MGC34413; T-lymphocyte activation antigen CD86

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基因别名: B7; B7-2; B7.2; B70; Cd28l2; Cd86; CLS1; ETC-1; Ly-58; Ly58; MB7; MB7-2; TS/A-2

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UniProt ID: (Mouse) P42082

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Entrez Gene ID: (Mouse) 12524

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功能

receptor binding immunoglobulin receptor superfamily

参与通路

toll-like receptor signaling pathway immune system process negative regulation of T cell anergy positive regulation of T cell proliferation positive regulation of activated T cell proliferation B cell activation defense response to virus cellular response to lipopolysaccharide