Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD88 (C5aR1) Monoclonal Antibody (20/70), PerCP-eFluor™ 710, eBioscience™
图: 1 / 2
CD88 (C5aR1) Antibody (46-0882-82) in Flow
Mouse bone marrow cells were stained with CD11b Monoclonal Antibody, PE (Product # 12-0112-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4031-82) (left) or 0.25 µg of CD88 (C5aR1) Monoclonal Antibody, PerCP-eFluor 710 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
产品信息
46-0882-82
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
20/70
抗原
RBL-2H3 transfected cells expressing murine C5aR
偶联物
激发/发射光谱
482/708 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2811808
产品详细信息
Description: This 20/70 monoclonal antibody recognizes mouse CD88 also known as C5aR1. CD88 is a complement receptor widely expressed on cells of the myeloid lineage. Clone 20/70 has been reported to block the binding of C5a to murine CD88.
Applications Reported: This 20/70 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 20/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
靶标信息
CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known. C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production. CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex. Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin receptor; C5a ligand; C5a Receptor; C5a-R; CD88; CD88 antigen; complement component 5, receptor 1
基因别名: C5aR; C5ar1; C5r1; Cd88; D7Msu1
UniProt ID: (Mouse) P30993
Entrez Gene ID: (Mouse) 12273
complement receptor mediated signaling pathway
apoptotic process
chemotaxis
signal transduction
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of vascular endothelial growth factor production
positive regulation of macrophage chemotaxis
cell proliferation in hindbrain
neutrophil chemotaxis
response to peptidoglycan
complement component C5a signaling pathway
mRNA transcription from RNA polymerase II promoter
negative regulation of neuron apoptotic process
positive regulation of angiogenesis
positive regulation of epithelial cell proliferation
defense response to Gram-positive bacterium
positive regulation of ERK1 and ERK2 cascade
positive regulation of neutrophil chemotaxis
