Invitrogen

CD8a Monoclonal Antibody (OKT8 (OKT-8)), FITC, eBioscience™

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CD8a Antibody (11-0086-42) in Flow

Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0199-42) and Mouse IgG2a kappa Isotype Control, FITC (Product # 11-4724-82) (left) or CD8a Monoclonal Antibody, FITC (right). Cells in the lymphocyte gate were used for analysis.

CD8a Antibody in Immunocytochemistry (ICC/IF)

CD8a Antibody in Immunoprecipitation (IP)

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产品信息

11-0086-42

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

查看26篇已发表文章 26篇已发表文章

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

OKT8 (OKT-8)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10598521

产品详细信息

Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Product # 14-0088 and clone HIT8a, Product # 14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.

Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins.

Cancer communications (London, England)

Qiao Y,Chen J,Wang X,Yan S,Tan J,Xia B,Chen Y,Lin K,Zou F,Liu B,He X,Zhang Y,Zhang X,Zhang H,Wu X,Lu L

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2023

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway.

Journal of experimental & clinical cancer research : CR

Liu Y,Xu Q,Deng F,Zheng Z,Luo J,Wang P,Zhou J,Lu X,Zhang L,Chen Z,Zhang Q,Chen Q,Zuo D

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2023

Single-cell profiling of the antigen-specific response to BNT162b2 SARS-CoV-2 RNA vaccine.

Nature communications

Kramer KJ,Wilfong EM,Voss K,Barone SM,Shiakolas AR,Raju N,Roe CE,Suryadevara N,Walker LM,Wall SC,Paulo A,Schaefer S,Dahunsi D,Westlake CS,Crowe JE,Carnahan RH,Rathmell JC,Bonami RH,Georgiev IS,Irish JM

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Thu Jun 16 00:00:00 UTC 2022

Myod1 and GR coordinate myofiber-specific transcriptional enhancers.

Nucleic acids research

Rovito D,Rerra AI,Ueberschlag-Pitiot V,Joshi S,Karasu N,Dacleu-Siewe V,Rayana KB,Ghaibour K,Parisotto M,Ferry A,Jelinsky SA,Laverny G,Klaholz BP,Sexton T,Billas IML,Duteil D,Metzger D

11-0086 was used in Flow cytometry/Cell sorting to investigate the skeletal muscle transcriptional program and identified key tissue-specific regulatory genetic elements.

Fri May 07 00:00:00 UTC 2021

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Wed Feb 23 00:00:00 UTC 2022

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Zhang S,Jiang VC,Han G,Hao D,Lian J,Liu Y,Zhang R,McIntosh J,Wang R,Dang M,Dai E,Wang Y,Santos D,Badillo M,Leeming A,Chen Z,Hartig K,Bigcal J,Zhou J,Kanagal-Shamanna R,Ok CY,Lee H,Steiner RE,Zhang J,Song X,Nair R,Ahmed S,Rodriquez A,Thirumurthi S,Jain P,Wagner-Bartak N,Hill H,Nomie K,Flowers C,Futreal A,Wang L,Wang M

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Mon May 17 00:00:00 UTC 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Mon Jul 26 00:00:00 UTC 2021

Clinical significance of CD38 and CD101 expression in PD-1⁺CD8⁺ T cells in patients with epithelial ovarian cancer.

Oncology letters

Zhou J,Wang W,Liang Z,Ni B,He W,Wang D

11-0086 was used in Flow cytometry/Cell sorting to show diagnosis of epithelial OC may be achieved by detecting co-expression of CD38 and CD101 in peripheral PD-1+CD8+ T cells.

Wed Jul 01 00:00:00 UTC 2020

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

11-0086 was used in Flow cytometry/Cell sorting to analyse T lymphocytes in patients' blood, to explore the T-cell-mediated component of systemic immunity.

Fri Mar 01 00:00:00 UTC 2019

Prognostic Values of CD38⁺CD101⁺PD1⁺CD8⁺ T Cells in Pancreatic Cancer.

Immunological investigations

Zhang M,Yang J,Zhou J,Gao W,Zhang Y,Lin Y,Wang H,Ruan Z,Ni B

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Mon Jul 01 00:00:00 UTC 2019

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Sun Apr 01 00:00:00 UTC 2018

Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.

Scientific reports

Reber AJ,Music N,Kim JH,Gansebom S,Chen J,York I

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Tue Apr 17 00:00:00 UTC 2018

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

11-0086 was used in Immunocytochemistry to indicate the enhanced anti-tumor efficacy of PD-1-deficient chimeric antigen receptor T cells against hepatocellular carcinoma.

Thu Sep 28 00:00:00 UTC 2023

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

11-0086 was used in Immunocytochemistry to indicate the enhanced anti-tumor efficacy of PD-1-deficient chimeric antigen receptor T cells against hepatocellular carcinoma.

Thu Sep 28 00:00:00 UTC 2023

CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1⁺ tumor cells, and extends the survival of tumor-bearing humanized mice.

Oncotarget

Horn LA,Ciavattone NG,Atkinson R,Woldergerima N,Wolf J,Clements VK,Sinha P,Poudel M,Ostrand-Rosenberg S

11-0086 was used in Flow cytometry/Cell sorting to suggest that the CD3xPDL1 BiTE may be efficacious for patients with PDL1(+) solid tumors, in combination with other immunotherapies that do not specifically neutralize PD1 pathway-mediated immune suppression.

Tue Aug 29 00:00:00 UTC 2017

CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1⁺ tumor cells, and extends the survival of tumor-bearing humanized mice.

Oncotarget

Horn LA,Ciavattone NG,Atkinson R,Woldergerima N,Wolf J,Clements VK,Sinha P,Poudel M,Ostrand-Rosenberg S

Tue Aug 29 00:00:00 UTC 2017

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

11-0086 was used in Flow cytometry/Cell sorting to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 UTC 2016

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Mon Feb 01 00:00:00 UTC 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Tue Mar 01 00:00:00 UTC 2016

Increased circulating IL-9-producing CD8⁺ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.

Experimental and therapeutic medicine

Wang W,Cheng ZS,Chen YF,Lin YH

Thu Dec 01 00:00:00 UTC 2016

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Immunofluorescence

Fri Jun 05 00:00:00 UTC 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Immunofluorescence

Fri Jun 05 00:00:00 UTC 2015

PD-1, PD-L1 and PD-L2 Gene Expression on T-Cells and Natural Killer Cells Declines in Conjunction with a Reduction in PD-1 Protein during the Intensive Phase of Tuberculosis Treatment.

PloS one

Hassan SS,Akram M,King EC,Dockrell HM,Cliff JM

11-0086 was used in Flow cytometry/Cell sorting to study PD-1 protein expression on the surface of various immune cells from tuberculosis patients.

Tue May 24 00:00:00 UTC 2016

Children with asthma by school age display aberrant immune responses to pathogenic airway bacteria as infants.

The Journal of allergy and clinical immunology

Larsen JM,Brix S,Thysen AH,Birch S,Rasmussen MA,Bisgaard H

11-0086 was used in Flow cytometry/Cell sorting to assess the immune responses to pathogen bacteria in children suffering from asthma.

Tue Apr 01 00:00:00 UTC 2014

TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.

PloS one

Reichwald K,Jørgensen TZ,Skov S

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Tue Nov 10 00:00:00 UTC 2015

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Mon Nov 11 00:00:00 UTC 2013

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

11-0086 was used in Flow cytometry/Cell sorting to understand the mechanisms by which IL-17 producing cells migrate into pathologic intervertebral disc tissues.

Fri Oct 13 00:00:00 UTC 2017

Modulation of NKG2D expression in human CD8(+) T cells corresponding with tuberculosis drug cure.

PloS one

Hassan SS,Cho JE,Akram M,Fielding KL,Dockrell HM,Cliff JM

11-0086 was used in Flow cytometry/Cell sorting to study expression patterns of the co-stimulation molecule NKG2D in human tuberculosis and assess changes during treatment.

Wed Apr 02 00:00:00 UTC 2014

Vaccine

Zhou X,McElhaney JE

11-0086 was used in Flow cytometry/Cell sorting to suggest that the cytolytic activity of CD4+ T cells responding to influenza is preserved with ageing, while that of effector memory and effector CD8+ T cells becomes diminished.

Thu Mar 03 00:00:00 UTC 2011

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.

Vaccine

Co MD,Orphin L,Cruz J,Pazoles P,Rothman AL,Ennis FA,Terajima M

Published figure using CD8a monoclonal antibody (Product # 11-0086-42) in Flow Cytometry

Mon Apr 07 00:00:00 UTC 2008

生物信息学

蛋白别名: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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基因别名: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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功能

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

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