Invitrogen

CD8a Monoclonal Antibody (OKT8 (OKT-8)), Biotin, eBioscience™

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CD8a Antibody (13-0086-82) in Flow

Staining of normal human peripheral blood cells with 0.06 µg of Mouse IgG2a kappa Isotype Control Biotin (Product # 13-4724-85) (open histogram) or 0.06 µg of Anti-Human CD8a Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

CD8a Antibody in Immunocytochemistry (ICC/IF)

CD8a Antibody in Immunoprecipitation (IP)

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产品信息

13-0086-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.06 µg/test

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免疫沉淀 (IP)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

OKT8 (OKT-8)

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_494040

产品详细信息

Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Product # 14-0088 and clone HIT8a, Product # 14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.

Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT8 (OKT-8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins.

Cancer communications (London, England)

Qiao Y,Chen J,Wang X,Yan S,Tan J,Xia B,Chen Y,Lin K,Zou F,Liu B,He X,Zhang Y,Zhang X,Zhang H,Wu X,Lu L

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2023

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway.

Journal of experimental & clinical cancer research : CR

Liu Y,Xu Q,Deng F,Zheng Z,Luo J,Wang P,Zhou J,Lu X,Zhang L,Chen Z,Zhang Q,Chen Q,Zuo D

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2023

Single-cell profiling of the antigen-specific response to BNT162b2 SARS-CoV-2 RNA vaccine.

Nature communications

Kramer KJ,Wilfong EM,Voss K,Barone SM,Shiakolas AR,Raju N,Roe CE,Suryadevara N,Walker LM,Wall SC,Paulo A,Schaefer S,Dahunsi D,Westlake CS,Crowe JE,Carnahan RH,Rathmell JC,Bonami RH,Georgiev IS,Irish JM

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Thu Jun 16 00:00:00 UTC 2022

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Wed Feb 23 00:00:00 UTC 2022

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Zhang S,Jiang VC,Han G,Hao D,Lian J,Liu Y,Zhang R,McIntosh J,Wang R,Dang M,Dai E,Wang Y,Santos D,Badillo M,Leeming A,Chen Z,Hartig K,Bigcal J,Zhou J,Kanagal-Shamanna R,Ok CY,Lee H,Steiner RE,Zhang J,Song X,Nair R,Ahmed S,Rodriquez A,Thirumurthi S,Jain P,Wagner-Bartak N,Hill H,Nomie K,Flowers C,Futreal A,Wang L,Wang M

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Mon May 17 00:00:00 UTC 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Mon Jul 26 00:00:00 UTC 2021

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2019

Prognostic Values of CD38⁺CD101⁺PD1⁺CD8⁺ T Cells in Pancreatic Cancer.

Immunological investigations

Zhang M,Yang J,Zhou J,Gao W,Zhang Y,Lin Y,Wang H,Ruan Z,Ni B

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Mon Jul 01 00:00:00 UTC 2019

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Sun Apr 01 00:00:00 UTC 2018

Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.

Scientific reports

Reber AJ,Music N,Kim JH,Gansebom S,Chen J,York I

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Tue Apr 17 00:00:00 UTC 2018

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Thu Sep 28 00:00:00 UTC 2023

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Tue Mar 01 00:00:00 UTC 2016

Increased circulating IL-9-producing CD8⁺ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.

Experimental and therapeutic medicine

Wang W,Cheng ZS,Chen YF,Lin YH

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2016

Oncogenic NRAS hyper-activates multiple pathways in human cord blood stem/progenitor cells and promotes myelomonocytic proliferation in vivo.

American journal of translational research

Wang T,Li C,Xia C,Dong Y,Yang D,Geng Y,Cai J,Zhang J,Zhang X,Wang J

13-0086 was used in Flow cytometry/Cell sorting to evaluate the effects of the NRASG12D mutation on human haematopoiesis and leukaemogenesis in vitro and in vivo.

Tue Dec 22 00:00:00 UTC 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Immunofluorescence

Fri Jun 05 00:00:00 UTC 2015

Structure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21.

The Journal of biological chemistry

Clairfeuille T,Norwood SJ,Qi X,Teasdale RD,Collins BM

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Immunofluorescence

Fri Jun 05 00:00:00 UTC 2015

TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.

PloS one

Reichwald K,Jørgensen TZ,Skov S

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Tue Nov 10 00:00:00 UTC 2015

Long-term immune reconstitution of naive and memory t cell pools after haploidentical hematopoietic stem cell transplantation.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Azevedo RI,Soares MV,Albuquerque AS,Tendeiro R,Soares RS,Martins M,Ligeiro D,Victorino RM,Lacerda JF,Sousa AE

13-0086 was used in Flow cytometry/Cell sorting to show that long-term immune reconstitution was successfully achieved after haploidentical hematopoietic stem cell transplantation, a process that appears to have largely relied on de novo T cell production.

Wed May 01 00:00:00 UTC 2013

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Mon Nov 11 00:00:00 UTC 2013

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Fri Oct 13 00:00:00 UTC 2017

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.

Vaccine

Co MD,Orphin L,Cruz J,Pazoles P,Rothman AL,Ennis FA,Terajima M

Published figure using CD8a monoclonal antibody (Product # 13-0086-82) in Flow Cytometry

Mon Apr 07 00:00:00 UTC 2008

生物信息学

蛋白别名: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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基因别名: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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功能

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

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