Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), Biotin, eBioscience™

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

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CD90.2 (Thy-1.2) Antibody (13-0903-82) in Flow

Staining of BALB/c splenocytes with 0.06 µg of Rat IgG2b K Isotype Control Biotin (Product # 13-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD90-2 (Thy-1-2) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD90.2 (Thy-1.2) Antibody in Immunocytochemistry (ICC/IF)

CD90.2 (Thy-1.2) Antibody in Immunohistochemistry (IHC)

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产品信息

13-0903-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.125 µg/test

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功能检测 (Functional)

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

30-H12

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_466536

产品详细信息

Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: The 30-H12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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A quiescent cell population replenishes mesenchymal stem cells to drive accelerated growth in mouse incisors.

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