Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (30-H12), PerCP-eFluor™ 710, eBioscience™

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

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CD90.2 (Thy-1.2) Antibody (46-0903-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.06 µg of Rat IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4031-82) (left) or 0.06 µg of Anti-Mouse CD90-2 (Thy-1-2) PerCP-eFluor® 710 (right). Total viable cells were used for analysis.

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产品信息

46-0903-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

30-H12

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10670882

产品详细信息

Description: The 30-H12 monoclonal antibody reacts with mouse CD90.2, also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/J, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 30-H12. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: This 30-H12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 30-H12 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence.

Nature communications

Chiang HY,Lu HH,Sudhakar JN,Chen YW,Shih NS,Weng YT,Shui JW

46-0903-82 was used in Flow Cytometry to conclude that IL-18 in return regulates Stat3-mediated anti-microbial response in Paneth cells, Akt-Tcf4-triggered expansion of Lgr5+ stem cells to facilitate tissue repair, and AIEC clearance by promoting IFNγ+ T cells.

Tue Feb 15 00:00:00 UTC 2022

Redox regulation of age-associated defects in generation and maintenance of T cell self-tolerance and immunity to foreign antigens.

Cell reports

Hester AK,Semwal MK,Cepeda S,Xiao Y,Rueda M,Wimberly K,Venables T,Dileepan T,Kraig E,Griffith AV

46-0903-82 was used in Flow Cytometry to find that diminished expression of a model tissue-restricted antigen gene in aged thymic stromal cells correlates with impaired clonal deletion of cognate T cells recognizing an autoantigen involved in atherosclerosis.

Tue Feb 15 00:00:00 UTC 2022

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 46-0903-82) in Flow Cytometry

Thu Jul 01 00:00:00 UTC 2021

Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders.

NPJ vaccines

Crouse B,Robinson C,Huseby Kelcher A,Laudenbach M,Abrahante JE,Pravetoni M

46-0903 was used in Flow cytometry/Cell sorting to find that blocking IL-4 improved the efficacy of vaccines targeting oxycodone and fentanyl in male and female mice.

Thu Oct 29 00:00:00 UTC 2020

Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders.

NPJ vaccines

Crouse B,Robinson C,Huseby Kelcher A,Laudenbach M,Abrahante JE,Pravetoni M

46-0903 was used in Flow cytometry/Cell sorting to find that blocking IL-4 improved the efficacy of vaccines targeting oxycodone and fentanyl in male and female mice.

Thu Oct 29 00:00:00 UTC 2020

GM-CSF Calibrates Macrophage Defense and Wound Healing Programs during Intestinal Infection and Inflammation.

Cell reports

Castro-Dopico T,Fleming A,Dennison TW,Ferdinand JR,Harcourt K,Stewart BJ,Cader Z,Tuong ZK,Jing C,Lok LSC,Mathews RJ,Portet A,Kaser A,Clare S,Clatworthy MR

46-0903 was used in Flow cytometry/Cell sorting to identify granulocyte-macrophage colony stimulating factor (GM-CSF) as a critical regulator of intestinal macrophage activation in patients with IBD and mice with dextran sodium sulfate (DSS)-induced colitis.

Tue Jul 07 00:00:00 UTC 2020

Functional Heterogeneity of Mouse Prostate Stromal Cells Revealed by Single-Cell RNA-Seq.

iScience

Kwon OJ,Zhang Y,Li Y,Wei X,Zhang L,Chen R,Creighton CJ,Xin L

46-0903 was used in Flow cytometry/Cell sorting to characterise mouse Prostate stromal cells heterogeneity by single-cell RNA-seq.

Fri Mar 29 00:00:00 UTC 2019

Dendritic cell function and pathogen-specific T cell immunity are inhibited in mice administered levonorgestrel prior to intranasal Chlamydia trachomatis infection.

Scientific reports

Quispe Calla NE,Vicetti Miguel RD,Mei A,Fan S,Gilmore JR,Cherpes TL

46-0903 was used in Flow cytometry/Cell sorting to demonstrate that levonorgestrel suppresses dendritic cell activation and function, and inhibits formation of pathogen-specific T-cell immunity.