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CD90.2 (Thy-1.2) Antibody (69-0902-82) in Flow
BALB/c mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, eFluor 506 (Product # 69-4321-82) (left) or 0.25 µg of CD90.2 Monoclonal Antibody, eFluor 506 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
产品信息
69-0902-82
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
53-2.1
偶联物
激发/发射光谱
419/508 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2762804
产品详细信息
Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Applications Reported: This 53-2.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor™ 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser
靶标信息
CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein
基因别名: CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2
UniProt ID: (Mouse) P01831
Entrez Gene ID: (Mouse) 21838
angiogenesis
negative regulation of protein kinase activity
cytoskeleton organization
single organismal cell-cell adhesion
negative regulation of cell migration
positive regulation of GTPase activity
retinal cone cell development
protein autophosphorylation
focal adhesion assembly
negative regulation of axonogenesis
T cell receptor signaling pathway
negative regulation of T cell receptor signaling pathway
positive regulation of T cell activation
positive regulation of release of sequestered calcium ion into cytosol
