Invitrogen

CD90 Monoclonal Antibody (F15-42-1)

CD90 Antibody in Immunocytochemistry (ICC/IF)

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CD90 Antibody (MA5-16671) in ICC/IF

Immunofluorescence analysis of CD90 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD90 Monoclonal Antibody (F15-42-1) (Product # MA5-16671) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pr... View More

CD90 Antibody in Immunohistochemistry (IHC)

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CD90 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD90 Monoclonal Antibody (F15-42-1) (Product # MA5-16671), shows relatively higher expression in SH-SY5Y cells as compared to MCF7 cells. {RE}

产品信息

MA5-16671

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100

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流式细胞分析 (Flow)

1:10-1:50

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免疫沉淀 (IP)

Assay-dependent

其他PubMed (Misc)

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产品规格

种属反应

Cynomolgus monkey, Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

F15-42-1

抗原

Purified human brain Thy-1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2538165

产品详细信息

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is human brain or thymus. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a approximately 25 kDa glycoprotein homologous to rat Thy1.

Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a approximately 25 kDa glycoprotein homologous to rat Thy1.

靶标信息

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Discovery of surface biomarkers for cell mechanophenotype via an intracellular protein-based enrichment strategy.

Cellular and molecular life sciences : CMLS

Dempsey ME,Chickering GR,González-Cruz RD,Fonseca VC,Darling EM

MA5-16671 was used in Flow cytometry/Cell sorting to demonstrate a workflow for surface protein discovery associated with cellular mechanophenotype.

Fri May 27 00:00:00 UTC 2022

Quantification of Antibody Persistence for Cell Surface Protein Labeling.

Cellular and molecular bioengineering

Dempsey ME,Woodford-Berry O,Darling EM

MA5-16671 was used in Immunocytochemistry-immunoflourescence to develop an easily adoptable method for researchers to characterize antibody removal timelines for any cell-antibody combination, with the benefit of studying broad, hypothesized mechanisms of antibody removal.

Tue Jun 01 00:00:00 UTC 2021

Lenvatinib Targets PDGFR-β Pericytes and Inhibits Synergy With Thyroid Carcinoma Cells: Novel Translational Insights.

The Journal of clinical endocrinology and metabolism

Iesato A,Li S,Roti G,Hacker MR,Fischer AH,Nucera C

MA5-16671 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation to demonstrate first-of-its-kind thyroid-specific model of lenvatinib therapeutic efficacy against pericyte viability, which disadvantages BRAFWT/V600E-PTC growth.

Fri Nov 19 00:00:00 UTC 2021

GLI1 and AXIN2 Are Distinctive Markers of Human Calvarial Mesenchymal Stromal Cells in Nonsyndromic Craniosynostosis.

International journal of molecular sciences

Di Pietro L,Barba M,Prampolini C,Ceccariglia S,Frassanito P,Vita A,Guadagni E,Bonvissuto D,Massimi L,Tamburrini G,Parolini O,Lattanzi W

MA5-16671 was used in Immunohistochemistry to analysing the expression of GLI1, AXIN2 and THY1 in the context of the human skull vault, using nonsyndromic forms of CS (NCS) as a model to test their functional implication in the aberrant osteogenic process.

Fri Jun 19 00:00:00 UTC 2020

GLI1 and AXIN2 Are Distinctive Markers of Human Calvarial Mesenchymal Stromal Cells in Nonsyndromic Craniosynostosis.

International journal of molecular sciences

Di Pietro L,Barba M,Prampolini C,Ceccariglia S,Frassanito P,Vita A,Guadagni E,Bonvissuto D,Massimi L,Tamburrini G,Parolini O,Lattanzi W

Fri Jun 19 00:00:00 UTC 2020

GLI1 and AXIN2 Are Distinctive Markers of Human Calvarial Mesenchymal Stromal Cells in Nonsyndromic Craniosynostosis.

International journal of molecular sciences

Di Pietro L,Barba M,Prampolini C,Ceccariglia S,Frassanito P,Vita A,Guadagni E,Bonvissuto D,Massimi L,Tamburrini G,Parolini O,Lattanzi W

Fri Jun 19 00:00:00 UTC 2020

Corneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with fibroblast growth factor-2, transforming growth factor-β3 and retinoic acid.

Journal of tissue engineering and regenerative medicine

Sidney LE,Hopkinson A

MA516671 was used in immunocytochemistry to explore factors that induce the transition of keratocytes to corneal mesenchymal stem cells and vice versa