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CD94 Antibody (17-5094-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) FITC (Product # 11-0569) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Canine/Human CD94 APC (right). Cells in the lymphocyte gate were used for analysis.
产品信息
17-5094-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
HP-3D9
偶联物
激发/发射光谱
651/660 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10854571
产品详细信息
Description: The HP-3D9 monoclonal antibody reacts with human CD94, a 70-kDa type II transmembrane glycoprotein. CD94 belongs to the C-type lectin superfamily and is expressed by NK cells, NKT cells, as well as subsets of CD8+ T cells and gamma delta T cells. This molecule is present as a heterodimer with members of the NKG2 family of receptors on the cell surface. CD94 dimerization with NKG2A results in an inhibitory receptor that suppresses NK cell function. In contrast, CD94 association with NKG2C and NKG2E leads to formation of an activating receptor complex that binds DAP12, an adapter protein that contains an ITAM motif. The CD94/NKG2 receptor complex binds the non-classical MHC Class Ib molecule HLA-E and plays important roles in adhesion and NK cell activation.
Crossblocking studies indicate that HP-3D9 and DX22 recognize the same epitope.
Applications Reported: This HP-3D9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HP-3D9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Natural killer (NK) cells, like CD94, are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD94; CD94 antigen; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KP43; natural killer cell receptor; Natural killer cells antigen CD94; NK cell receptor
基因别名: CD94; KLRD1
UniProt ID: (Dog) Q38HS3, (Human) Q13241
Entrez Gene ID: (Dog) 611360, (Human) 3824
