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产品信息
14-0959-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human
已发表种属
Human,
Mouse,
Rhesus monkey
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
DX2
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_467397
产品详细信息
Description: The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.
Applications Reported: The DX2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. DX2 has also been reported in in vitro studies of Fas-induced apoptosis. EOS9.1 is recommended for induction of apoptosis in vitro .
Applications Tested: The DX2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6
基因别名: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6
UniProt ID: (Human) P25445
Entrez Gene ID: (Dog) 486469, (Human) 355
ovarian follicle atresia
immunoglobulin production
renal system process
protein complex assembly
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
activation-induced cell death of T cells
inflammatory cell apoptotic process
inflammatory response
immune response
signal transduction
spermatogenesis
brain development
aging
circadian rhythm
extrinsic apoptotic signaling pathway via death domain receptors
response to toxic substance
gene expression
regulation of gene expression
B cell mediated immunity
telencephalon development
dendrite regeneration
positive regulation of protein homooligomerization
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
response to drug
ovulation cycle
regulation of apoptotic process
chordate embryonic development
positive regulation of apoptotic process
negative regulation of apoptotic process
regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
response to peptide hormone
negative thymic T cell selection
regulation of lymphocyte differentiation
regulation of myeloid cell differentiation
response to cycloheximide
spleen development
negative regulation of B cell activation
protein homooligomerization
response to glucocorticoid
maternal process involved in female pregnancy
positive regulation of lymphocyte apoptotic process
cellular response to hydrogen peroxide
response to growth factor
cellular response to phenylalanine
cellular response to mechanical stimulus
cellular response to cobalt ion
cellular response to lithium ion
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to estrogen stimulus
cellular response to hyperoxia
cellular response to hypoxia
cellular response to hydrostatic pressure
motor neuron apoptotic process
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
hepatocyte apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
liver regeneration
necroptotic signaling pathway
regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
response to fluoride
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
