Invitrogen

CDCA8 Polyclonal Antibody

CDCA8 Antibody in Immunocytochemistry (ICC/IF)

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CDCA8 Antibody (PA5-55771) in ICC/IF

Immunofluorescent staining of CDCA8 in human cell line U-2 OS using a CDCA8 Polyclonal Antibody (Product # PA5-55771) shows localization to nucleus, nucleoli and cytokinetic bridge.

CDCA8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CDCA8 Antibody in Immunohistochemistry (IHC)

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CDCA8 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of CDCA8 demonstrates antibody specificity. Immunohistochemical analysis of CDCA8 using anti-CDCA8 Polyclonal Antibody (Product # PA5-55771), shows significant staining of CDCA8 in human testis and shows minimal or weak staining in human endometrium tissues. The relative expression levels of CDCA8 within each tissue is shown using RNA-Seq. {RE}

产品信息

PA5-55771

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human CDCA8. Recombinant protein control fragment (Product #RP-94215).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2639578

产品详细信息

Immunogen sequence: PLKSAKTRKV IQVDEMIVEE EEEEENERKN LQTARVKRCP PSKKRTQSIQ GKGKGKRSSR ANTVTPAVGR LEVSMVKPTP GLTPRFDSR

Highest antigen sequence identity to the following orthologs: Mouse - 64%, Rat - 66%.

靶标信息

CDCA8 is a component of a chromosomal passenger complex (CPC) required for stability of the bipolar mitotic spindle. The chromosomal passenger complex, which includes Survivin, CDCA8, INCENP and Aurora-B, is known to play crucial roles during mitosis and cell division. It was found that CDCA8 interacting with Aurora-B, INCENP and Survivin, increases during G2/M phase and then reduces after exit from M phase. CDCA8 is cell cycle regulated, down-regulated in response to p53/Rb-signaling, and up-regulated in many types of cancerous tissues. In Drosophila cells, inactivation of CDCA8 results in polyploidy, delayed mitosis and abnormal tissue development, indicating its critical role for cell proliferation. Recent studies show that CDCA8 is essential for cell proliferation during early embryonic development, and its early embryonic lethality cannot be rescued by the loss of p53. Its aberrant expression is linked to a poor prognosis for gastric cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。