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CDH11 Antibody (PA5-96937) in WB
Western blot analysis of CDH11 in extracts of various cell lines. Samples were incubated with CDH11 Polyclonal antibody (Product # PA5-96937) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-96937
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 120-400 of human CDH11 (NP_0017882)
偶联物
形式
Liquid
浓度
0.63 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808739
产品详细信息
Positive Samples: U-87MG, A-549, HT-29, SKOV3, Mouse lung, Mouse spleen; Cellular Location: Cell membrane, Single-pass type I membrane protein
Immunogen sequence: ERAQYTLMAQ AVDRDTNRPL EPPSEFIVKV QDINDNPPEF LHETYHANVP ERSNVGTSVI QVTASDADDP TYGNSAKLVY SILEGQPYFS VEAQTGIIRT ALPNMDREAK EEYHVVIQAK DMGGHMGGLS GTTKVTITLT DVNDNPPKFP QSVYQMSVSE AAVPGEEVGR VKAKDPDIGE NGLVTYNIVD GDGMESFEIT TDYETQEGVI KLKKPVDFET KRAYSLKVEA ANVHIDPKFI SNGPFKDTVT VKISVEDADE PPMFLAPSYI HEVQENAAAG T
靶标信息
This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. All cadherins are single-pass transmembrane proteins with a variable number of 110 amino acid extracellular cadherin (EC) domains. Classical cadherins contain five large N-terminal EC domains, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Based on amino acid alignment, classical cadherins are divided into type I and type II subgroups. Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) cadherin differ from type I cadherins in their specific amino acid sequences. Type II cadherins include human cadherin-5, -6, -8, -11, and -12. Type II cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Cadherin-11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme. Strong expression of Cadherin-11 has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. A variant Cadherin-11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins. The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence. Recently, Cadherin-11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested but not in non-invasive cell lines, suggesting that Cadherin-11 may be a marker for aggressive, invasive tumor subsets.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cadherin; cadherin 11, type 2, OB-cadherin (osteoblast); Cadherin-11; OB Cadherin; OB-cadherin; OSF-4; Osteoblast cadherin; osteoblast-cadherin
基因别名: Cad-11; CAD11; CDH11; CDHOB; OB; OSF-4
UniProt ID: (Human) P55287, (Mouse) P55288
Entrez Gene ID: (Human) 1009, (Rat) 84407, (Mouse) 12552
