Invitrogen

CDK9 Recombinant Rabbit Monoclonal Antibody (SD204-07)

CDK9 Antibody in Immunocytochemistry (ICC/IF)

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CDK9 Antibody (MA5-32397) in ICC/IF

Immunofluorescence analysis of Cyclin-dependent kinase 9 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CDK9 Recombinant Rabbit Monoclonal Antibody (SD204-07) (Product # MA5-32397) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Second... View More

CDK9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CDK9 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Cyclin-dependent kinase 9 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-CDK9 Recombinant Rabbit Monoclonal Antibody (SD204-07) (Product # MA5-32397). {KD}

产品信息

MA5-32397

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SD204-07

抗原

Synthetic peptide within Human Cdk9 aa 323-372

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809675

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

CDK9 is a serine/threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1. CDK9 regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: C-2K; CDC2-related kinase; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Cyclin-dependent kinase 9; RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit

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基因别名: C-2k; CDC2L4; CDK9; CTK1; PITALRE; TAK

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UniProt ID: (Human) P50750

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Entrez Gene ID: (Human) 1025

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