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CDO1 Antibody (PA5-104298) in ICC/IF
Immunocytochemistry analysis of CDO1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CDO1 polyclonal antibody (Product # PA5-104298) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104298
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CDO1(Accession Q16878), corresponding to amino acid residues V142-A192.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2853608
Product Specific Information
Antibody detects endogenous levels of total CDO1.
Target Information
CDO1 (cysteine dioxygenase, type I) belongs to the cysteine dioxygenase family and is involved in organosulfur biosynthesis. CDO1 functions as a dioxygenase that uses iron and zinc as cofactors to catalyze the conversion of L-cysteine and oxygen to 3-sulfinoalanine. CDO1 exists as a monomer and is expressed at high levels in liver and placenta and at lower levels in brain, pancreas and heart. CDO1 is involved in pyruvate-, sulfate- and taurine-related metabolic pathways and is a crucial regulator of cysteine concentrations within the cell.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDO; CDO-I; CDOI; cysteine dioxygenase 1, cytosolic; Cysteine dioxygenase type 1; Cysteine dioxygenase type I; cysteine dioxygenase, type I; cytosolic cysteine dioxygenase 1
Gene Aliases: 1300002L19Rik; Cdo; CDO-I; CDO1; D18Ucla3
UniProt ID: (Human) Q16878, (Rat) P21816, (Mouse) P60334
Entrez Gene ID: (Human) 1036, (Rat) 81718, (Mouse) 12583
sulfur amino acid biosynthetic process
cysteine metabolic process
inflammatory response
lactation
L-cysteine catabolic process
L-cysteine catabolic process to pyruvate, using cysteine dioxygenase
response to glucagon
taurine biosynthetic process
response to amino acid
response to ethanol
response to glucocorticoid
response to cAMP
oxidation-reduction process
response to organonitrogen compound
L-cysteine metabolic process
L-cysteine catabolic process to taurine
taurine metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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