Invitrogen

CDT1 Polyclonal Antibody

CDT1 Antibody in Immunocytochemistry (ICC/IF)

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CDT1 Antibody (PA5-18088) in ICC/IF

Immunofluorescence analysis of CDT1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CDT1 Polyclonal Antibody (Product # PA5-18088) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11078) at a... View More

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CDT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-2 OS cells were transfected with CDT1 siRNA and reduction of signal was observed in Western Blot using CDT1 Polyclonal Antibody (Product # PA5-18088). {KD}

产品信息

PA5-18088

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (ARLAHQTRAEEGL) corresponding to the C-terminus amino acids of CDT1 (aa 534-546).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_10985522

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

靶标信息

KMT2A is a histone methyltransferase that plays an essential role in early development and hematopoiesis. It is a catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, KMT2A specifically mediates H3K4me, a tag for epigenetic transcriptional activation. KMT2A has weak methyltransferase activity by itself, and requires other components of the MLL1/MLL complex to obtain full methyltransferase activity. It has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', and has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9 and promotes PPP1R15A-induced apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。