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CENPA Antibody (MA1-20832) in ICC/IF
Immunofluorescence analysis of CENPA was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CENPA Monoclonal Antibody (3-19) (Product # MA1-20832) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766... View More
产品信息
MA1-20832
应用
建议稀释比
已发表文章
产品规格
种属反应
Chicken,
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
3-19
抗原
Synthetic peptide corresponding to residues P(3) R R R S R K P E A P R R R S P S(19) of human CENPA protein.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2, with 50% glycerol
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2078763
产品详细信息
Recommended positive controls: , BE cells.
This antibody does not cross react with mouse CENPA proteins.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CENP A; CENP-A; Centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; centromere-specific histone; ggCENP-A; Histone H3-like centromeric protein A
基因别名: CenH3; CENP-A; CENPA
UniProt ID: (Human) P49450, (Chicken) Q6XXM1
Entrez Gene ID: (Human) 1058, (Chicken) 100113359
