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CENPA Antibody (MA5-47616) in ICC/IF
Immunofluorescence analysis of Centromere protein A (CENPA) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CENPA Recombinant Rabbit Monoclonal Antibody (PSH0-09) (Product # MA5-47616) at 1:100 dilution in 0.1% BSA, incubated at 4 °C overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, ... View More
产品信息
MA5-47616
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH0-09
抗原
Synthetic peptide within human cenpa aa 21-70 / 140.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2942597
产品详细信息
No Cross with Mouse and Rat.
Expression varies across the cell cycle, with high levels in G2 phase (at the mRNA level).
Positive control: Hela cell lysate, A375 cell lysate.
Subcellular location: Nucleus, Chromosome, centromere, kinetochore.
靶标信息
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CENP A; CENP-A; Centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; centromere-specific histone; Histone H3-like centromeric protein A
基因别名: CenH3; CENP-A; CENPA
UniProt ID: (Human) P49450
Entrez Gene ID: (Human) 1058
