Invitrogen

CENPB Monoclonal Antibody (A5F5)

CENPB Antibody in Immunocytochemistry (ICC/IF)

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CENPB Antibody (MA5-42558) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of CENPB in A549 cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with CENPB Monoclonal Antibody (Product # MA5-42558) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

CENPB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-42558

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:50-1:100

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

A5F5

抗原

Recombinant protein within human CENPB aa 1-200/599

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2911699

产品详细信息

Positive Control: Hela cell lysate, A549 cell lysate, MCF-7 cell lysate, A549, human kidney tissue, human skin tissue.

Subcellular Location: Nucleus, centromere.

靶标信息

CENPB is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes and is considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

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