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CENPF Antibody (MA1-23185) in ICC/IF
Immunofluorescence analysis of CENPF was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CENPF Monoclonal Antibody (Product # MA1-23185) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A32723) at a dilution of 1:2000 for 45 minutes at ro... View More
产品信息
MA1-23185
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
14C10 1D8
抗原
Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_560120
产品详细信息
Recommended positive controls: HeLa (G2 phase).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitosis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: AH antigen; cell-cycle-dependent 350K nuclear protein; CENP-F; CENP-F kinetochore protein; Centromere protein F; centromere protein F, 350/400ka (mitosin); centromere protein F, 350/400kDa (mitosin); Kinetochore protein CENPF; Mitosin; RP11-262H5.1
基因别名: CENF; CENPF; CILD31; hcp-1; PRO1779; STROMS
UniProt ID: (Human) P49454
Entrez Gene ID: (Human) 1063
mitotic cell cycle
kidney development
chromosome segregation
sister chromatid cohesion
mitotic nuclear division
mitotic spindle assembly checkpoint
muscle organ development
cell proliferation
regulation of G2/M transition of mitotic cell cycle
protein transport
regulation of striated muscle tissue development
ventricular system development
cell differentiation
response to drug
negative regulation of transcription, DNA-templated
cell division
metaphase plate congression
kinetochore assembly
regulation of cell cycle
DNA biosynthetic process
