Invitrogen

CEP170 Monoclonal Antibody (72-413-1)

CEP170 Antibody in Immunocytochemistry (ICC/IF)

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CEP170 Antibody (41-3200) in ICC/IF

Immunofluorescence analysis of CEP170 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CEP170 (72-413-1) Mouse Monoclonal Antibody (Product # 41-3200) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # ... View More

CEP170 Antibody in Immunoprecipitation (IP)

CEP170 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CEP170 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

CEP170 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

CEP170 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

41-3200

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 12 publications 12 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

72-413-1

Immunogen

Recombinant protein derived from the N-terminal region of the human Cep170 protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533502

Target Information

The product of this gene is a component of the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes. The protein interacts with and is phosphorylated by polo-like kinase 1, and functions in maintaining microtubule organization and cell morphology. The human genome contains a putative transcribed pseudogene. Several alternatively spliced transcript variants of this gene have been found, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Self-assembly of pericentriolar material in interphase cells lacking centrioles.

eLife

Chen F,Wu J,Iwanski MK,Jurriens D,Sandron A,Pasolli M,Puma G,Kromhout JZ,Yang C,Nijenhuis W,Kapitein LC,Berger F,Akhmanova A

41-3200 was used in Immunocytochemistry-immunofluorescence to reveal that interphase PCM contains a set of components that can self-assemble into a compact structure and organize microtubules, but PCM self-organization is sensitive to motor- and microtubule-based rearrangement.

Tue Jul 05 00:00:00 UTC 2022

Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.

Cell reports

Ho JJD,Cunningham TA,Manara P,Coughlin CA,Arumov A,Roberts ER,Osteen A,Kumar P,Bilbao D,Krieger JR,Lee S,Schatz JH

Published figure using CEP170 monoclonal antibody (Product # 41-3200) in Western Blot

Tue Oct 12 00:00:00 UTC 2021

Cep128 associates with Odf2 to form the subdistal appendage of the centriole.

Genes to cells : devoted to molecular & cellular mechanisms

Kashihara H,Chiba S,Kanno SI,Suzuki K,Yano T,Tsukita S

Published figure using CEP170 monoclonal antibody (Product # 41-3200) in Immunocytochemistry

Fri Mar 01 00:00:00 UTC 2019

Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis.

Cell

Hilgendorf KI,Johnson CT,Mezger A,Rice SL,Norris AM,Demeter J,Greenleaf WJ,Reiter JF,Kopinke D,Jackson PK

41-3200 was used in Immunocytochemistry to investigate whether preadipocytes use their cilia to sense and respond to external cues to remodel white adipose tissue.

Wed Nov 27 00:00:00 UTC 2019

Centriole Overduplication is the Predominant Mechanism Leading to Centrosome Amplification in Melanoma.

Molecular cancer research : MCR

Denu RA,Shabbir M,Nihal M,Singh CK,Longley BJ,Burkard ME,Ahmad N

41-3200 was used in Immunohistochemistry-immunofluorescence to assess the relative contributions of failure of cell division and cell-cell fusion with.

Thu Mar 01 00:00:00 UTC 2018

Centriole Overduplication is the Predominant Mechanism Leading to Centrosome Amplification in Melanoma.

Molecular cancer research : MCR

Denu RA,Shabbir M,Nihal M,Singh CK,Longley BJ,Burkard ME,Ahmad N

41-3200 was used in Immunohistochemistry-immunofluorescence to assess the relative contributions of failure of cell division and cell-cell fusion with.

Thu Mar 01 00:00:00 UTC 2018

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Developmental cell

Kanie T,Abbott KL,Mooney NA,Plowey ED,Demeter J,Jackson PK

41-3200 was used in Immunocytochemistry-immunoflourescence to identify CEP350, FOP, and the RABL2B GTPase as the proteins organising the first known mechanism directing ciliary entry of intraflagellar transport complexes.

Mon Jul 10 00:00:00 UTC 2017

Nesprin-1α-Dependent Microtubule Nucleation from the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells.

Current biology : CB

Gimpel P,Lee YL,Sobota RM,Calvi A,Koullourou V,Patel R,Mamchaoui K,Nédélec F,Shackleton S,Schmoranzer J,Burke B,Cadot B,Gomes ER

41-3200 was used in Immunocytochemistry to identify a novel function for Nesprin-1α/Nesprin-1 in nuclear positioning through recruitment of Akap450-mediated microtubule nucleation activity to the nuclear envelope.

Mon Oct 09 00:00:00 UTC 2017

Nesprin-1α-Dependent Microtubule Nucleation from the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells.

Current biology : CB

Gimpel P,Lee YL,Sobota RM,Calvi A,Koullourou V,Patel R,Mamchaoui K,Nédélec F,Shackleton S,Schmoranzer J,Burke B,Cadot B,Gomes ER

Mon Oct 09 00:00:00 UTC 2017

Nesprin-1α-Dependent Microtubule Nucleation from the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells.

Current biology : CB

Gimpel P,Lee YL,Sobota RM,Calvi A,Koullourou V,Patel R,Mamchaoui K,Nédélec F,Shackleton S,Schmoranzer J,Burke B,Cadot B,Gomes ER

Mon Oct 09 00:00:00 UTC 2017

Nesprin-1α-Dependent Microtubule Nucleation from the Nuclear Envelope via Akap450 Is Necessary for Nuclear Positioning in Muscle Cells.

Current biology : CB

Gimpel P,Lee YL,Sobota RM,Calvi A,Koullourou V,Patel R,Mamchaoui K,Nédélec F,Shackleton S,Schmoranzer J,Burke B,Cadot B,Gomes ER

Mon Oct 09 00:00:00 UTC 2017

CDKL5 localizes at the centrosome and midbody and is required for faithful cell division.

Scientific reports

Barbiero I,Valente D,Chandola C,Magi F,Bergo A,Monteonofrio L,Tramarin M,Fazzari M,Soddu S,Landsberger N,Rinaldo C,Kilstrup-Nielsen C

Published figure using CEP170 monoclonal antibody (Product # 41-3200) in Immunofluorescence

Mon Jul 24 00:00:00 UTC 2017

STAR syndrome-associated CDK10/Cyclin M regulates actin network architecture and ciliogenesis.

Cell cycle (Georgetown, Tex.)

Guen VJ,Gamble C,Perez DE,Bourassa S,Zappel H,Gärtner J,Lees JA,Colas P

41-3200 was used in immunocytochemistry to learn regulation of actin network architecture and ciliogenesis due to STAR syndrome-associated CDK10/Cyclin M

Thu Dec 15 00:00:00 UTC 2016

14-3-3γ Prevents Centrosome Amplification and Neoplastic Progression.

Scientific reports

Mukhopadhyay A,Sehgal L,Bose A,Gulvady A,Senapati P,Thorat R,Basu S,Bhatt K,Hosing AS,Balyan R,Borde L,Kundu TK,Dalal SN

41-3200 was used in immunocytochemistry to analyze prevention of centrosome amplification and neoplastic progression by 14-3-3gamma

Thu Jun 02 00:00:00 UTC 2016

14-3-3γ Prevents Centrosome Amplification and Neoplastic Progression.

Scientific reports

Mukhopadhyay A,Sehgal L,Bose A,Gulvady A,Senapati P,Thorat R,Basu S,Bhatt K,Hosing AS,Balyan R,Borde L,Kundu TK,Dalal SN

41-3200 was used in immunocytochemistry to analyze prevention of centrosome amplification and neoplastic progression by 14-3-3gamma

Thu Jun 02 00:00:00 UTC 2016

Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis.

Nature communications

Pillai S,Nguyen J,Johnson J,Haura E,Coppola D,Chellappan S

41-3200 was used in immunocytochemistry, immunoprecipitation, and western blot to determine how Tank binding kinase 1 works in mitosis and microtubule dynamiccs

Thu Dec 10 00:00:00 UTC 2015

Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis.

Nature communications

Pillai S,Nguyen J,Johnson J,Haura E,Coppola D,Chellappan S

41-3200 was used in immunocytochemistry, immunoprecipitation, and western blot to determine how Tank binding kinase 1 works in mitosis and microtubule dynamiccs

Thu Dec 10 00:00:00 UTC 2015

Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis.

Nature communications

Pillai S,Nguyen J,Johnson J,Haura E,Coppola D,Chellappan S

41-3200 was used in immunocytochemistry, immunoprecipitation, and western blot to determine how Tank binding kinase 1 works in mitosis and microtubule dynamiccs

Thu Dec 10 00:00:00 UTC 2015

Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis.

Nature communications

Pillai S,Nguyen J,Johnson J,Haura E,Coppola D,Chellappan S

41-3200 was used in immunocytochemistry, immunoprecipitation, and western blot to determine how Tank binding kinase 1 works in mitosis and microtubule dynamiccs

Thu Dec 10 00:00:00 UTC 2015

A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis.

PLoS genetics

Fuchs M,Luthold C,Guilbert SM,Varlet AA,Lambert H,Jetté A,Elowe S,Landry J,Lavoie JN

41-3200 was used in western blot to characterize the role of BAG3-HSPB8 in mitosis

Thu Oct 01 00:00:00 UTC 2015

Link between DNA damage and centriole disengagement/reduplication in untransformed human cells.

Journal of cellular physiology

Douthwright S,Sluder G

41-3200 was used in immunocytochemistry to examine centriole behavior after DNA damage in synchronized untransformed human cells.

Wed Oct 01 00:00:00 UTC 2014

PACSIN proteins bind tubulin and promote microtubule assembly.

Experimental cell research

Grimm-Günter EM,Milbrandt M,Merkl B,Paulsson M,Plomann M

Tue Jun 10 00:00:00 UTC 2008

PACSIN proteins bind tubulin and promote microtubule assembly.

Experimental cell research

Grimm-Günter EM,Milbrandt M,Merkl B,Paulsson M,Plomann M

Tue Jun 10 00:00:00 UTC 2008

PACSIN proteins bind tubulin and promote microtubule assembly.

Experimental cell research

Grimm-Günter EM,Milbrandt M,Merkl B,Paulsson M,Plomann M

Tue Jun 10 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: centrosomal protein 170kDa; Centrosomal protein of 170 kDa; Cep170; KARP-1-binding protein; KARP1-binding protein; XRCC5 binding protein

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Gene Aliases: CEP170; FAM68A; KAB; KIAA0470

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UniProt ID: (Human) Q5SW79

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Entrez Gene ID: (Human) 9859

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Function(s)

protein binding scaffold/adaptor protein

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CEP170 Monoclonal Antibody (72-413-1)

CEP170 Antibody (41-3200) in ICC/IF

Product Details

41-3200

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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