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FIGURE: 1 / 1
CES3 Antibody (PA5-92252) in WB
Western blot analysis of CES3 in extracts of various cell lines. Samples were incubated with CES3 Polyclonal antibody (Product # PA5-92252) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Product Details
PA5-92252
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 269-568 of human CES3 (NP_0011721061)
Conjugate
Form
Liquid
Concentration
0.71 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806421
Product Specific Information
Immunogen sequence: WPLAQKIANT LACSSSSPAE MVQCLQQKEG EELVLSKKLK NTIYPLTVDG TVFPKSPKEL LKEKPFHSVP FLMGVNNHEF SWLIPRGWGL LDTMEQMSRE DMLAISTPVL TSLDVPPEMM PTVIDEYLGS NSDAQAKCQA FQEFMGDVFI NVPTVSFSRY LRDSGSPVFF YEFQHRPSSF AKIKPAWVKA DHGAEGAFVF GGPFLMDESS RLEATEEEKQ LSLTMMAQWT HFARTGDPNS KALPPWPQFN QAEQYLEINP VPRAGQKFRE AWMQFWSETL PSKIQQWHQK QKNRKAQEDL; Positive Samples: LO2, HT-29, Mouse small intestine, Mouse large intestine, Rat liver, Rat small intestine; Cellular Location: Endoplasmic reticulum lumen
Target Information
CES3 major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decreasing acyl chain length of the fatty acid moiety. Catalyzes the synthesis of fatty acid ethyl esters.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carboxyesterase ES-10; Carboxylesterase 1D; Carboxylesterase 3; carboxylesterase 3 (brain); carboxylesterase 3, pseudogene 1; Carboxylesterase 3A; EC 3.1.1; EC 3.1.1.1; ES-HVEL; ES-male; Es-male carboxylesterase; ES31FLJ21736; esterase 31; Esterase-31; FAEE synthase; Fatty acid ethyl ester synthase; Liver carboxylesterase 10; Liver carboxylesterase 31; Liver carboxylesterase 31 homolog; pI 6.1 esterase; TGH; Triacylglycerol hydrolase
Gene Aliases: Ces1; Ces1d; CES3; Ces3-ps1; Ces31; Ces3a; ES31; TGH; UNQ869/PRO1887
UniProt ID: (Human) Q6UWW8, (Mouse) Q8VCT4, (Rat) P16303, (Mouse) Q63880
Entrez Gene ID: (Human) 23491, (Mouse) 104158, (Rat) 113902, (Mouse) 382053, (Rat) 689754
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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