Invitrogen

CFTR Polyclonal Antibody

CFTR Antibody in Immunocytochemistry (ICC/IF)

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CFTR Antibody (PA1-935) in ICC/IF

Immunolocalization of CFTR in HEK293 cells stably transfected with the CFTR gene.

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产品信息

PA1-935

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1.0 µg/mL

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: G(103) R I I A S Y D P D N K E E R(117)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2081228

产品详细信息

PA1-935 detects cystic fibrosis transmembrane conductance factor (CFTR) from cells overexpressing the human protein.

PA1-935 has been successfully used in immunocytochemistry procedures. Immunocytochemical staining of HEK293 cells overexpressing human CFTR with this antibody results in staining primarily of the plasma membrane.

PA1-935 immunizing peptide corresponds to amino acid residues 103-117 from human CFTR protein. This sequence is completely conserved between human, rabbit, and monkey and there is a one amino acid substitution in rat, bovine, and sheep.

靶标信息

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Incomplete reprogramming after fusion of human multipotent stromal cells and bronchial epithelial cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Curril IM,Koide M,Yang CH,Segal A,Wellman GC,Spees JL

PA1-935 was used in immunocytochemistry to investigate cell function of hybrid cells from genetically tagged multipotent stromal cells and normal human bronchial epithelial cells

Wed Dec 01 00:00:00 UTC 2010

Disruption of CFTR-dependent lipid rafts reduces bacterial levels and corneal disease in a murine model of Pseudomonas aeruginosa keratitis.

Investigative ophthalmology & visual science

Zaidi T,Bajmoczi M,Zaidi T,Golan DE,Pier GB

PA1-935 was used in immunocytochemistry to study the mechanisms for the entrance of Pseudomonas aeruginosa into human corneal epithelial cells and the ways to disrupt Pseudomonas aeruginosa infection.

Sat Mar 01 00:00:00 UTC 2008

Annexin A5 increases the cell surface expression and the chloride channel function of the DeltaF508-cystic fibrosis transmembrane regulator.

Biochimica et biophysica acta

Le Drévo MA,Benz N,Kerbiriou M,Giroux-Metges MA,Pennec JP,Trouvé P,Férec C

PA1-935 was used in immunocytochemistry to study the effect of annexin A5 on the expression and of the deltaF508-cystic fibrosis transmembrane regulator

Wed Oct 01 00:00:00 UTC 2008

生物信息学

蛋白别名: ATP Binding Cassette Superfamily C Member 7 (ABCC7); ATP-binding cassette sub-family C member 7; cAMP-dependent chloride channel; CFTR; Channel conductance-controlling ATPase; Cystic fibrosis transmembrane conductance regulator; cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7); tcag7.78; TNR CFTR

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基因别名: ABC35; ABCC7; CF; CFTR; CFTR/MRP; dJ760C5.1; MRP7; TNR-CFTR

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UniProt ID: (Human) P13569

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Entrez Gene ID: (Human) 1080

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功能