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CHCHD4 Antibody (PA5-89016) in WB
Western blot analysis of CHCHD4 in extracts of various cell lines. Samples were incubated with CHCHD4 Polyclonal antibody (Product # PA5-89016) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
产品信息
PA5-89016
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human CHCHD4 (NP_0010919721)
偶联物
形式
Liquid
浓度
1.71 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805299
产品详细信息
Immunogen sequence: MSYCRQEGKD RIIFVTKEDH ETPSSAELVA DDPNDPYEEH GLILPNGNIN WNCPCLGGMA SGPCGEQFKS AFSCFHYSTE EIKGSDCVDQ FRAMQECMQK YPDLYPQEDE DEEEEREKKP AEQAEETAPI EATATKEEEG SS; Positive Samples: LO2, 293T, Mouse liver, Mouse heart, Mouse pancreas; Cellular Location: Mitochondrion intermembrane space
靶标信息
CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Coiled-coil-helix-coiled-coil-helix domain-containing protein 4; mitochondrial intermembrane space import and assembly 40 homolog; Mitochondrial intermembrane space import and assembly protein 40; translocase of inner mitochondrial membrane 40 homolog
基因别名: 2410012P20Rik; 2810014D17Rik; AI838740; CHCHD4; MIA40; TIMM40
UniProt ID: (Human) Q8N4Q1, (Mouse) Q8VEA4, (Rat) Q5BJN5
Entrez Gene ID: (Human) 131474, (Mouse) 72170, (Rat) 312559
transport
protein transport
protein maturation by protein folding
cellular response to oxidative stress
mitochondrial DNA repair
protein import into mitochondrial intermembrane space
regulation of protein export from nucleus
'de novo' posttranslational protein folding
oxidation-reduction process
establishment of protein localization to mitochondrion
positive regulation of cellular respiration
