Invitrogen

CHD4 Polyclonal Antibody

CHD4 Antibody in Immunocytochemistry (ICC/IF)

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CHD4 Antibody (PA5-81154) in ICC/IF

Immunofluorescence staining of CHD4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CHD4 Polyclonal Antibody (Product # PA5-81154, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

CHD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CHD4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. K-562 cells were transfected with CHD4 siRNA and decrease in signal intensity was observed in Western Blot application using CHD4 Polyclonal Antibody (Product # PA5-81154). {KD}

产品信息

PA5-81154

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:300-1:10,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to the C-terminus of the Human CHD4

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2788391

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human CHD4.

靶标信息

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。