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CHP2 Antibody (PA5-101475) in IHC (P)
Immunohistochemistry analysis of CHP2 in human liver cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CHP2 polyclonal antibody (Product # PA5-101475) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
产品信息
PA5-101475
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human CHP2(Accession O43745), corresponding to amino acid residues P103-L153.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2850910
产品详细信息
Antibody detects endogenous levels of total CHP2.
靶标信息
CHP2 (Calcineurin B homologous protein 2), also known as Hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains four EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Calcineurin B homologous protein 2; calcineurin-like EF hand protein 2; calcineurin-like EF-hand protein 2; Chp-2; hepatocellular carcinoma antigen gene 520; Hepatocellular carcinoma-associated antigen 520; Hepatocellular carcinoma-associated antigen 520 homolog
基因别名: 2010110P09Rik; Cbhp2; chp-2; CHP2; HCA520
UniProt ID: (Human) O43745, (Rat) Q810D1, (Mouse) Q9D869
Entrez Gene ID: (Human) 63928, (Rat) 308965, (Mouse) 70261
cellular sodium ion homeostasis
positive regulation of cell proliferation
positive regulation of phosphatase activity
protein transport
regulation of cellular pH
positive regulation of protein import into nucleus
positive regulation of transcription from RNA polymerase II promoter
positive regulation of calcineurin-NFAT signaling cascade
cellular response to calcium ion
transport
sodium ion transport
regulation of pH
