Invitrogen

CHRM2 Monoclonal Antibody (31-1D1)

CHRM2 Antibody in Immunocytochemistry (ICC/IF)

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CHRM2 Antibody (MA3-044) in ICC/IF

Immunofluorescence analysis of Muscarinic Acetylcholine Receptor M2 was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled Muscarinic Acetylcholine Receptor M2 (31-1D1) Mouse Monoclonal Antibody (Product # MA3-044) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary... View More

CHRM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA3-044

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Pig, Rat

已发表种属

Human, Pig, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

31-1D1

抗原

Purified porcine cardiac m2 muscarinic acetylcholine receptor.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2080073

产品详细信息

MA3-044 detects muscarinic 2 acetylcholine receptor (m2AChR) from human, mouse, pig and rat tissues. This antibody does not detect mAChR from chicken tissues. MA3-044 is specific for the m2 mAChR subtype.

MA3-044 has been successfully used in Western blot and immunoprecipitation protocols. MA3-044 is an ~52kDa protein; however, by Western blot this antibody may detect 60-80 kDa protein(s) (depending on the degree of glycosylation) representing the m2AChR from pig heart.

The MA3-044 antigen is purified porcine cardiac m2 AChR.

靶标信息

Muscarinic cholinergic receptor 2 (CHRM2) is an Acetylcholine Receptor has been suggested to function as a presynaptic autoreceptor that inhibits acetylcholine release. The M2 receptor has also been suggested to mediate smooth muscle contractility. Alzheimer's disease (AD) involves selective loss of M2 subtype receptors in cortical and hippocampal regions of the human brain. Increase in M2 receptor heterozygosity has been associated with depression in women. The M2 receptor has been reported to be expressed in various regions of the brain including the cerebellum, cerebral cortex, hippocampus, medulla, striatum, and thalamus, and in lung and prostate.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Enhanced Contractive Tension and Upregulated Muscarinic Receptor 2/3 in Colorectum Contribute to Constipation in 6-Hydroxydopamine-Induced Parkinson's Disease Rats.

Frontiers in aging neuroscience

Zhang XL,Zhang XH,Yu X,Zheng LF,Feng XY,Liu CZ,Quan ZS,Zhang Y,Zhu JX

MA3-044 was used in Immunohistochemistry, Western Blot to study the effect of central dopaminergic denervation in rats, induced by injection 6-hydroxydopamine into the bilateral substania nigra, on colorectal contractive activity, content of acetylcholine, vasoactive intestinal peptide and expression of neural nitric oxide synthase and muscarinic receptor.

Tue Jan 11 00:00:00 UTC 2022

Enhanced Contractive Tension and Upregulated Muscarinic Receptor 2/3 in Colorectum Contribute to Constipation in 6-Hydroxydopamine-Induced Parkinson's Disease Rats.

Frontiers in aging neuroscience

Zhang XL,Zhang XH,Yu X,Zheng LF,Feng XY,Liu CZ,Quan ZS,Zhang Y,Zhu JX

Tue Jan 11 00:00:00 UTC 2022

Smooth muscle adaptation and recovery of contractility after massive small bowel resection in rats.

Experimental biology and medicine (Maywood, N.J.)

Chen J,Wen J,Cai W

MA3-044 was used in western blot to study muscarinic M2 and M3 receptor expression and smooth muscle contractility during recovery from major small bowel resection

Tue May 01 00:00:00 UTC 2012

Cholesterol as a determinant of cooperativity in the M2 muscarinic cholinergic receptor.

Biochemical pharmacology

Colozo AT,Park PS,Sum CS,Pisterzi LF,Wells JW

MA3-044 was used in western blot to investigate the role of M2 muscarinic cholinergic receptors

Sun Jul 15 00:00:00 UTC 2007

Cooperativity manifest in the binding properties of purified cardiac muscarinic receptors.

The Journal of biological chemistry

Wreggett KA,Wells JW

MA3-044 was used in western blot to characterize the binding capability of muscarinic receptors

Fri Sep 22 00:00:00 UTC 1995

生物信息学

蛋白别名: 7TM receptor; acetylcholine receptor; acetylcholine receptor, muscarinic 2; AChR M2; cardiac muscarinic acetylcholine receptor; cholinergic receptor; cholinergic receptor, muscarinic 2; cholinergic receptor, muscarinic 2, cardiac; cholinergic receptor, muscarinic 2, isoform a; FLJ43243; M2 muscarinic acetylcholine receptor; M2-mAChR; m2AChR; MGC120006; MGC120007; muscarinic acetylcholine receptor 2; Muscarinic acetylcholine receptor M2; muscarinic M2 receptor; muscarinic receptor m2; unnamed protein product

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基因别名: AChR-M2; Acm2; Chrm-2; CHRM2; HM2; M2