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CHRNA9 Antibody (PA5-121250) in WB
Western blot analysis of CHRNA9 in extracts of various cell lines. Samples were incubated with CHRNA9 Polyclonal antibody (Product # PA5-121250) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-121250
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-240 of human CHRNA9 (NP_060051.2).
Conjugate
Form
Liquid
Concentration
0.78 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914822
Product Specific Information
Positive test controls include: B cells, HeLa, HT-1080, Jurkat, Mouse thymus, Rat brain, Rat thymus. The target is usually found in the following locations: Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse.
Immunogen sequence: ADGKYAQKLF NDLFEDYSNA LRPVEDTDKV LNVTLQITLS QIKDMDERNQ ILTAYLWIRQ IWHDAYLTWD RDQYDGLDSI RIPSDLVWRP DIVLYNKADD ESSEPVNTNV VLRYDGLITW DAPAITKSSC VVDVTYFPFD NQQCNLTFGS WTYNGNQVDI FNALDSGDLS DFIEDVEWEV HGMPAVKNVI SYGCCSEPYP DVTFTLLLKR RSSFY
Target Information
CHRNA9 is a member of the ligand-gated ionic channel family and nicotinic acetylcholine receptor superfamily. CHRNA9 is a plasma membrane protein that forms homo- or hetero-oligomeric divalent cation channels. It is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea.This gene is a member of the ligand-gated ionic channel family and nicotinic acetylcholine receptor gene superfamily. It encodes a plasma membrane protein that forms homo- or hetero-oligomeric divalent cation channels. This protein is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea. The protein is additionally expressed in keratinocytes, the pituitary gland, B-cells and T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: acetylcholine receptor; acetylcholine receptor alpha 9 subunit (nAChR); acetylcholine receptor, neuronal nicotinic, alpha-9 subunit; cholinergic receptor; cholinergic receptor, nicotinic alpha 9; cholinergic receptor, nicotinic, alpha 9 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 9; NACHR alpha 9; NACHR alpha-9; neuronal acetylcholine receptor protein, alpha-9 subunit; Neuronal acetylcholine receptor subunit alpha-9; nicotinic acetylcholine receptor subunit alpha 9; Nicotinic acetylcholine receptor subunit alpha-9
Gene Aliases: 2410015I05Rik; Acra9; CHRNA9; EG666827; Gm8311; HSA243342; NACHRA9
UniProt ID: (Human) Q9UGM1, (Rat) P43144
Entrez Gene ID: (Human) 55584, (Mouse) 231252, (Rat) 65024
signal transduction
positive regulation of cytosolic calcium ion concentration
synaptic transmission, cholinergic
inner ear morphogenesis
detection of mechanical stimulus involved in sensory perception of sound
calcium ion transmembrane transport
cation transport
cation transmembrane transport
sensory perception of sound
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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