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FIGURE: 1 / 1
CHST1 Antibody (PA5-88951) in WB
Western blot analysis of CHST1 in extracts of various cell lines. Samples were incubated with CHST1 Polyclonal antibody (Product # PA5-88951) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-88951
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 62-411 of human CHST1 (NP_0036451)
Conjugate
Form
Liquid
Concentration
2.39 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805247
Product Specific Information
Immunogen sequence: HILILATTRS GSSFVGQLFN QHLDVFYLFE PLYHVQNTLI PRFTQGKSPA DRRVMLGASR DLLRSLYDCD LYFLENYIKP PPVNHTTDRI FRRGASRVLC SRPVCDPPGP ADLVLEEGDC VRKCGLLNLT VAAEACRERS HVAIKTVRVP EVNDLRALVE DPRLNLKVIQ LVRDPRGILA SRSETFRDTY RLWRLWYGTG RKPYNLDVTQ LTTVCEDFSN SVSTGLMRPP WLKGKYMLVR YEDLARNPMK KTEEIYGFLG IPLDSHVARW IQNNTRGDPT LGKHKYGTVR NSAATAEKWR FRLSYDIVAF AQNACQQVLA QLGYKIAASE EELKNPSVSL VEERDFRPFS; Positive Samples: Mouse liver, Mouse kidney, Rat brain; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein
Target Information
Carbohydrate sulfotransferase 1 (CHST1), also referred to as KSGal6ST or KSST, is a keratan sulfate sulfotransferase. It is predominantly expressed in brain and skeletal muscle and localizes to the trans-Golgi network. CHST1 is responsible for mediating the sulfation of keratan in the cornea, which is important in maintaining corneal transparency. In particular, CHST1 catalyzes the transfer of sulfate groups from 3'-phosphoadenosine 5'-phosphosulfate to position six of internal or terminal galactose residues (preferentially the residues adjacent to sulfated GlcNAc) on growing keratan sulfate chains. CHST1 also contributes to the generation of L-selectin ligands. Mutations in the CHST1 gene may play a role in macular corneal dystrophy (MCD).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Carbohydrate sulfotransferase 1; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; GST-1; Keratan sulfate Gal-6 sulfotransferase; KS6ST; KSGal6ST; KSST; N-acetylglucosamine-6-O-sulfotransferase 1
Gene Aliases: 2610008E20Rik; AW125896; C6ST; Chst1; GST-1; Gst1; KSGAL6ST
UniProt ID: (Rat) Q5RJQ0, (Mouse) Q9EQC0
Entrez Gene ID: (Rat) 295934, (Mouse) 76969
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