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CHST15 Antibody (PA5-97171) in WB
Western blot analysis of CHST15 in extracts of various cell lines. Samples were incubated with CHST15 Polyclonal antibody (Product # PA5-97171) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-97171
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 452-561 of human CHST15 (NP_0569762)
Conjugate
Form
Liquid
Concentration
1.77 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808973
Product Specific Information
Immunogen sequence: LQVGLYAVYL LDWLSVFDKQ QFLILRLEDH ASNVKYTMHK VFQFLNLGPL SEKQEALMTK SPASNARRPE DRNLGPMWPI TQKILRDFYR PFNARLAQVL ADEAFAWKTT; Positive Samples: Raji, Rat heart; Cellular Location: Golgi apparatus membrane, Single-pass type II membrane protein
Target Information
GALNAC4S-6ST is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4, 6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. GALNAC4S-6ST may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell RAG associated protein; B cell RAG associated protein (GALNAC4S-6ST); B-cell RAG-associated gene protein; Carbohydrate sulfotransferase 15; DKFZp781H1369; GalNAc4S-6ST; hBRAG; MGC34346; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; RP11-47G11.1
Gene Aliases: BRAG; CHST15; GALNAC4S-6ST; GALNAC4S6ST; KIAA0598
UniProt ID: (Human) Q7LFX5, (Rat) Q8CHI9
Entrez Gene ID: (Human) 51363, (Rat) 286974
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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