Invitrogen

CIC Polyclonal Antibody

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CIC Antibody (PA1-46018) in WB

Western blot analysis of CIC in HeLa: 5, 15, and 50 µg; 293T: 50 µg whole cell lysates of HeLa and 293T. Samples were incubated in CIC polyclonal antibody (Product # PA1-46018) using a dilution of 1 µg/mL.

CIC Antibody in Immunoprecipitation (IP)

CIC Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

产品信息

PA1-46018

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,500

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

2-5 µg/mg lysate

染色质免疫沉淀实验 (ChIP)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide made to a region within residues 1500-1608 of the human Capicua protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2291736

产品详细信息

By Western blot, PA1-46018 is specific for the ~250 kDa CIC protein.

CIC (~164 kDa) interacts with Ataxin 1 (~87 kDa).

This may explain the band at 250 kDa (complex).Suggested positive control: Hela whole cell extract.

靶标信息

This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Global and precise identification of functional miRNA targets in mESCs by integrative analysis.

EMBO reports

Schaefer M,Nabih A,Spies D,Hermes V,Bodak M,Wischnewski H,Stalder P,Ngondo RP,Liechti LA,Sajic T,Aebersold R,Gatfield D,Ciaudo C

Published figure using CIC polyclonal antibody (Product # PA1-46018) in Western Blot

Mon Sep 05 00:00:00 UTC 2022

Capicua suppresses YAP1 to limit tumorigenesis and maintain drug sensitivity in human cancer.

Cell reports

Kim JW,Luck C,Wu W,Ponce RK,Lin YK,Gupta N,Okimoto RA

PA1-46018 was used in ChIP Assay and Western Blotting to show that the ERK-regulated transcription factor CIC can directly repress YAP1 expression through non-consensus GGAAGGAA DNA-binding motifs in a proximal YAP1 regulatory element.

Tue Oct 04 00:00:00 UTC 2022

Capicua suppresses YAP1 to limit tumorigenesis and maintain drug sensitivity in human cancer.

Cell reports

Kim JW,Luck C,Wu W,Ponce RK,Lin YK,Gupta N,Okimoto RA

Tue Oct 04 00:00:00 UTC 2022

The CIC-ERF co-deletion underlies fusion-independent activation of ETS family member, ETV1, to drive prostate cancer progression.

eLife

Gupta N,Song H,Wu W,Ponce RK,Lin YK,Kim JW,Small EJ,Feng FY,Huang FW,Okimoto RA

PA1-46018 was used in Western Blotting to show that two neighboring transcription factors, Capicua (CIC) and ETS2 repressor factor (ERF), which are co-deleted in human prostate tumors can drive prostate oncogenesis.

Wed Nov 16 00:00:00 UTC 2022

The CIC-ERF co-deletion underlies fusion-independent activation of ETS family member, ETV1, to drive prostate cancer progression.

eLife

Gupta N,Song H,Wu W,Ponce RK,Lin YK,Kim JW,Small EJ,Feng FY,Huang FW,Okimoto RA

Wed Nov 16 00:00:00 UTC 2022

Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro.

Frontiers in neuroanatomy

Li K,Shao S,Ji T,Liu M,Wang L,Pang Y,Chen M,Xu S,Zhang K,Wang Q,Zhuang Z,Wei L,Zhang Y,Chen Y,Wang Y,Zhang J,Chen K,Lian H,Zhong C

PA1-46018 was used in Immunocytochemistry, Western Blot to investigate the function of Capicua, a transcriptional repressor frequently mutated in brain cancer oligodendroglioma, in the dendritic growth of neurons in the hippocampus.

Sat Aug 14 00:00:00 UTC 2021

Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro.

Frontiers in neuroanatomy

Li K,Shao S,Ji T,Liu M,Wang L,Pang Y,Chen M,Xu S,Zhang K,Wang Q,Zhuang Z,Wei L,Zhang Y,Chen Y,Wang Y,Zhang J,Chen K,Lian H,Zhong C

Sat Aug 14 00:00:00 UTC 2021

Capicua Regulates Dendritic Morphogenesis Through Ets in Hippocampal Neurons in vitro.

Frontiers in neuroanatomy

Li K,Shao S,Ji T,Liu M,Wang L,Pang Y,Chen M,Xu S,Zhang K,Wang Q,Zhuang Z,Wei L,Zhang Y,Chen Y,Wang Y,Zhang J,Chen K,Lian H,Zhong C

Sat Aug 14 00:00:00 UTC 2021

Extracellular signal-regulated kinase mediates chromatin rewiring and lineage transformation in lung cancer.

eLife

Inoue Y,Nikolic A,Farnsworth D,Shi R,Johnson FD,Liu A,Ladanyi M,Somwar R,Gallo M,Lockwood WW

Published figure using CIC polyclonal antibody (Product # PA1-46018) in Western Blot

Mon Jun 14 00:00:00 UTC 2021

Capicua regulates neural stem cell proliferation and lineage specification through control of Ets factors.

Nature communications

Ahmad ST,Rogers AD,Chen MJ,Dixit R,Adnani L,Frankiw LS,Lawn SO,Blough MD,Alshehri M,Wu W,Marra MA,Robbins SM,Cairncross JG,Schuurmans C,Chan JA

PA1-46018 was used in Immunohistochemistry-immunofluorescence to demonstrate that forebrain-specific Cic deletion increases proliferation and self-renewal of neural stem cells.

Wed May 01 00:00:00 UTC 2019

生物信息学

蛋白别名: Capicua; capicua homolog; Protein capicua homolog

查看更多收起

基因别名: 1200010B10Rik; CIC; KIAA0306; mKIAA0306

查看更多收起

UniProt ID: (Human) Q96RK0, (Mouse) Q924A2

查看更多收起

Entrez Gene ID: (Human) 23152, (Dog) 484475, (Rat) 308435, (Mouse) 71722

查看更多收起

功能

DNA binding chromatin binding protein binding

参与通路

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated lung alveolus development

CIC Polyclonal Antibody

CIC Antibody (PA1-46018) in WB

产品信息

PA1-46018

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

CIC Polyclonal Antibody

CIC Antibody (PA1-46018) in WB

产品信息

PA1-46018

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)