Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 9
CIP2A Antibody (MA1-46001) in ICC/IF
Immunofluorescence analysis of CIP2A was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CIP2A Monoclonal Antibody (2G10) (Product # MA1-46001) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti- Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A2817... View More
产品信息
MA1-46001
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
已发表种属
Human
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
2G10
抗原
A C-terminal portion of the human p90 protein.
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2130794
产品详细信息
Suggested positive control: Hela whole cell extract, antigen standard for KIAA1524 (transient overexpression lysate).
靶标信息
Inhibition of protein phosphatase 2A (PP2A) activity has been identified as a prerequisite for the transformation of human cells. The protein, designated Cancerous Inhibitor of PP2A (CIP2A), interacts directly with the oncogenic transcription factor c-Myc, inhibits PP2A activity toward c-Myc serine 62 (S62), and thereby prevents c-Myc proteolytic degradation. In addition to its function in c-Myc stabilization, CIP2A promotes anchorage-independent cell growth and in vivo tumor formation. The oncogenic activity of CIP2A is demonstrated by transformation of human cells by overexpression of CIP2A. Importantly, CIP2A is overexpressed in two common human malignancies, head and neck squamous cell carcinoma (HNSCC) and colon cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cancerous inhibitor of PP2A; cancerous inhibitor of protein phosphatase 2A; p90 autoantigen; p90 autoantigen homolog; Protein CIP2A; similar to RIKEN cDNA C330027C09
基因别名: AA408511; AU018569; CIP2A; KIAA1524; p90
UniProt ID: (Mouse) Q8BWY9, (Human) Q8TCG1
Entrez Gene ID: (Mouse) 224171, (Human) 57650, (Rat) 360711
