Invitrogen

CISD2 Polyclonal Antibody

CISD2 Antibody in Immunohistochemistry (IHC)

图: 1 / 11

CISD2 Antibody (PA5-34545) in IHC

Immunohistochemistry of CISD2 in rat brain tissue with CISD2 Polyclonal Antibody (Product # PA5-34545) at 2.5 µg/mL.

Product Image Gallery

CISD2 Antibody — Figure 3 Curcumin upregulated CISD2 protein expression in vivo. ( A ) CISD2 protein expression in mouse spinal cords following intraperitoneal administration of curcumin at a concentration of 40 mg/kg. The upper panel presents the results of immunoblotting analysis of CISD2 (15 kDa) with GAPDH (37 kDa) serving as an internal control. The lower panel presents the mean +- (SEM) of CISD2/GAPDH band intensity as a ratio of the results from the control group (n = 4). Curcumin enhanced CISD2 mRNA expression in vitro via JAK/STAT signaling pathways. mRNA expression of CISD2 ( B ) and iNOS ( C ) in neuron-like cells (SH-SY5Y). mRNA expression levels of CISD2 ( D ) and iNOS ( E ) in astrocyte culture. ( F ) CISD2 mRNA expression following treatment with curcumin and the inhibition of JAK/STAT signaling pathways in non-stimulated astrocytes: AG490: JAK/STAT inhibitor, LY294002: PI3K inhibitor, RO318220: PKC inhibitor, U0126: MAPK inhibitor. Vertical bars indicate the mean + (SEM) of mRNA expression (n = 3). * p < 0.05, and ##,** p < 0.01 indicate differences of statistical significance. {TM}

产品信息

PA5-34545

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

2.5 µg/mL

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 15 amino acid peptide near the center of human CISD2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2551897

产品详细信息

A suggested positive control is rat brain tissue lysate.

PA5-34545 can be used with blocking peptide PEP-1588.

靶标信息

Defects in the CISD2 (CDGSH iron sulfur domain 2) gene are a cause of the neurodegenerative disorder Wolfram syndrome 2. CISD2 is a zinc finger protein that localizes to the endoplasmic reticulum and mitochondria and binds an iron/sulfur cluster. CISD2 interacts with Bcl-2 and can be displaced by the BH3-only protein BIK and contributes to the regulation of BIK-initiated autophagy. CISD2 deficiency in mice causes mitochondrial breakdown accompanied by autophagic cell death as well as the development of premature aging phenotype.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Anti-Inflammatory CDGSH Iron-Sulfur Domain 2: A Biomarker of Central Nervous System Insult in Cellular, Animal Models and Patients.

Biomedicines

Kung WM,Lin CC,Chen WJ,Jiang LL,Sun YY,Hsieh KH,Lin MS

Published figure using CISD2 polyclonal antibody (Product # PA5-34545) in Immunohistochemistry

Sun Mar 27 00:00:00 UTC 2022

Anti-Inflammatory CDGSH Iron-Sulfur Domain 2: A Biomarker of Central Nervous System Insult in Cellular, Animal Models and Patients.

Biomedicines

Kung WM,Lin CC,Chen WJ,Jiang LL,Sun YY,Hsieh KH,Lin MS

Published figure using CISD2 polyclonal antibody (Product # PA5-34545) in Immunohistochemistry

Sun Mar 27 00:00:00 UTC 2022

CISD2 Attenuates Inflammation and Regulates Microglia Polarization in EOC Microglial Cells-As a Potential Therapeutic Target for Neurodegenerative Dementia.

Frontiers in aging neuroscience

Lin MS

PA5-34545 was used in Western Blotting to investigate whether CISD2 attenuation influences anti-inflammatory effects and M1-M2 polarization in microglia.

Sat Oct 03 00:00:00 UTC 2020

CISD2 Attenuates Inflammation and Regulates Microglia Polarization in EOC Microglial Cells-As a Potential Therapeutic Target for Neurodegenerative Dementia.

Frontiers in aging neuroscience

Lin MS

PA5-34545 was used in Western Blotting to investigate whether CISD2 attenuation influences anti-inflammatory effects and M1-M2 polarization in microglia.

Sat Oct 03 00:00:00 UTC 2020

Wild Bitter Melon Exerts Anti-Inflammatory Effects by Upregulating Injury-Attenuated CISD2 Expression following Spinal Cord Injury.

Behavioural neurology

Kung WM,Lin CC,Kuo CY,Juin YC,Wu PC,Lin MS

PA5-34545 was used in Western Blotting to understand the effects of agents found in wild bitter melon, including PPAR-beta and alpha-ESA, on spinal cord injury mouse models.

Wed Aug 18 00:00:00 UTC 2021

Wild Bitter Melon Exerts Anti-Inflammatory Effects by Upregulating Injury-Attenuated CISD2 Expression following Spinal Cord Injury.

Behavioural neurology

Kung WM,Lin CC,Kuo CY,Juin YC,Wu PC,Lin MS

PA5-34545 was used in Western Blotting to understand the effects of agents found in wild bitter melon, including PPAR-beta and alpha-ESA, on spinal cord injury mouse models.

Wed Aug 18 00:00:00 UTC 2021

Protective Effects of CISD2 and Influence of Curcumin on CISD2 Expression in Aged Animals and Inflammatory Cell Model.

Nutrients

Lin CC,Chiang TH,Sun YY,Lin MS

PA5-34545 was used in Western Blotting to investigate the role of CISD2 and how curcumin regulates CISD2 in the aging process.

Mon Mar 25 00:00:00 UTC 2019

A novel CISD2 mutation associated with a classical Wolfram syndrome phenotype alters Ca2+ homeostasis and ER-mitochondria interactions.

Human molecular genetics

Rouzier C,Moore D,Delorme C,Lacas-Gervais S,Ait-El-Mkadem S,Fragaki K,Burté F,Serre V,Bannwarth S,Chaussenot A,Catala M,Yu-Wai-Man P,Paquis-Flucklinger V

Published figure using CISD2 polyclonal antibody (Product # PA5-34545) in Western Blot

Mon May 01 00:00:00 UTC 2017

生物信息学

蛋白别名: CDGSH iron sulfur domain-containing protein 2; CDGSH iron-sulfur domain-containing protein 2; Endoplasmic reticulum intermembrane small protein; Miner1; mitoNEET related 1; MitoNEET-related 1 protein; NAF-1; nervous system over-expressed protein 70; Nervous system overexpressed protein 70; Nutrient-deprivation autophagy factor-1; zinc finger, CDGSH-type domain 2

查看更多收起

基因别名: 1500009M05Rik; 1500026J14Rik; 1500031D15Rik; AI848398; B630006A20Rik; CDGSH2; CISD2; ERIS; Miner1; NAF-1; Noxp70; RGD1566242; WFS2; ZCD2

查看更多收起

UniProt ID: (Human) Q8N5K1, (Mouse) Q9CQB5

查看更多收起

Entrez Gene ID: (Human) 493856, (Rat) 295457, (Mouse) 67006

查看更多收起

功能

protein binding protein homodimerization activity poly(A) RNA binding metal ion binding 2 iron, 2 sulfur cluster binding iron-sulfur cluster binding

参与通路

mitophagy multicellular organism aging regulation of autophagy autophagy