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CLASP2 Antibody (MA5-47709) in ICC/IF
Immunocytochemistry analysis of CLASP2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha-Tubulin (left) and rat CLASP2 monoclonal antibody (Product # MA5-47709) (middle). The antibodies were detected using either goat anti-mouse: DyLight® 488 or goat anti-Rat: DyLight® 594.
产品信息
MA5-47709
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rat
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
KT68
抗原
Clone KT68 was generated from a GST fusion protein containing the N-terminus of mouse CLASP2. This sequence is highly conserved in human and rat CLASP2, and has low homology to CLASP1.
偶联物
形式
Liquid
浓度
~1.0 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2942690
产品详细信息
The antibody detects a 160 kDa* doublet corresponding to the molecular mass of CLASP2 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects CLASP2 by immunocytochemistry in HeLa and A431 cells.
靶标信息
CLASP2 is microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CLASP2alpha; CLASP2gamma; CLIP associating protein 2; CLIP-associating protein 2; CLIP-associating protein CLASP2; Cytoplasmic linker-associated protein 2; hOrbit2; multiple asters (Mast)-like homolog 2; Protein Orbit homolog 2
基因别名: 1500004F14Rik; 8030404L10Rik; C77448; CLASP2; CLASP2beta; KIAA0627; mKIAA0627
UniProt ID: (Human) O75122, (Mouse) Q8BRT1, (Rat) Q99JD4
Entrez Gene ID: (Human) 23122, (Mouse) 76499, (Rat) 114514
microtubule cytoskeleton organization
vesicle targeting
microtubule nucleation
negative regulation of microtubule depolymerization
Golgi organization
mitotic spindle organization
sister chromatid cohesion
establishment or maintenance of cell polarity
exit from mitosis
regulation of gastrulation
positive regulation of epithelial cell migration
regulation of epithelial to mesenchymal transition
microtubule organizing center organization
regulation of microtubule polymerization or depolymerization
regulation of microtubule-based process
microtubule anchoring
positive regulation of exocytosis
cell division
negative regulation of stress fiber assembly
negative regulation of focal adhesion assembly
establishment of protein localization to plasma membrane
positive regulation of extracellular matrix disassembly
negative regulation of wound healing, spreading of epidermal cells
positive regulation of basement membrane assembly involved in embryonic body morphogenesis
cell cycle
mitotic nuclear division
cell migration
meiotic cell cycle
