Invitrogen

CLBT Polyclonal Antibody

CLBT Antibody in Immunocytochemistry (ICC/IF)

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CLBT Antibody (PA5-144664) in ICC/IF

Immunocytochemistry analysis of CLBT in SiHa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with CLBT polyclonal antibody (Product # PA5-144664) with a dilution of 1:100 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Nuclear DNA was labelled in blue w... View More

CLBT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-144664

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化（石蜡） (IHC (P))

1:600

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein within human CLTB aa 30-229/229.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3093524

产品详细信息

Positive control: MCF-7 cell lysate, Hela cell lysate, 293T cell lysate, human placenta tissue lysate, rat testis tissue, SiHa.

Predicted band size: 25 kDa

Subcellular Location: Cytoplasmic vesicle membrane, Membrane, coated pit.

靶标信息

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

仅用于科研。不用于诊断过程。未经明确授权不得转售。